Differences in G-actin containing bound ATP or ADP: the Mg2+-induced conformational change requires ATP.
Differences in G-actin containing bound ATP or ADP: the Mg2+-induced conformational change requires ATP.
复制标题
含有结合 ATP 或 ADP 的 G 肌动蛋白的差异：Mg2 诱导的构象变化需要 ATP。
DOI：
10.1021/bi00336a056
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Patane,K
中科院分区：
文献类型：
--
作者：
Frieden,C;Patane,K
材料与方法酵母己糖激酶和ATP（99-100%纯）获自Sigma。N-（1-芘基）碘乙酰胺得自Molecule Probes，A-（碘乙酰基）-A-（5-磺基-1-萘基）乙二胺得自Pierce Chemical Co.“超纯”MgS 04获自Alzón Corp.，阿尔法分局所有其他材料均为试剂级，并且所有溶液均用去离子蒸馏水制备。1缩写：1，5-IAEDANS标记的G-肌动蛋白，用iV-标记的肌动蛋白，（碘乙酰基）-A '-（5-磺基-1-萘基）乙二胺;芘标记的G-肌动蛋白，7V-标记的肌动蛋白（1-芘基）碘乙酰胺; Tris-HCl，三-（羟甲基）氨基甲烷盐酸盐; G缓冲液，2 mM Tris-HCl，pH 8，含有200 µ ATP、200 µ Ca 2+和1.5 mM NaN 3; EGTA，乙二醇双（O-氨基乙基醚）-MMA。W '-四乙酸。
Materials and MethodsMaterials. Yeast hexokinase and ATP (99-100% pure) were obtained from Sigma. N-(1-Pyrenyl) iodoacetamide was obtained from Molecule Probes and A-(iodoacetyl)-A-(5-sulfo-1-naphthyl) ethylenediaminefrom Pierce Chemical Co.“Ultrapure” MgSQ4 was obtained from Ventrón Corp., Alpha Division. All other materials were reagent grade, and all solutions were made with deionized distilled water.1 Abbreviations: 1, 5-IAEDANS-labeled G-actin, actin labeled with iV-(iodoacetyl)-A'-(5-sulfo-1-naphthyl) ethylenediamine; pyrene-labeled G-actin, actin labeled with 7V-(l-pyrenyl) iodoacetamide; Tris-HCl, tris-(hydroxymethyl) aminomethane hydrochloride; G buffer, 2 mM Tris-HCl, pH 8, containing 200 µ ATP, 200 µ Ca2+, and 1.5 mM NaN3; EGTA, ethylene glycol bis (0-aminoethyl etherl-MMA'. W'-tetraacetic acid.