Self-Assembled Chromogenic Polymeric Nanoparticle-Laden Nanocarrier as a Signal Carrier for Derivative Binary Responsive Virus Detection
Self-Assembled Chromogenic Polymeric Nanoparticle-Laden Nanocarrier as a Signal Carrier for Derivative Binary Responsive Virus Detection
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DOI：
10.1021/acsami.1c08813
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发表时间：
2021-07-30
影响因子：
9.5
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
--
作者：
Khoris, Indra Memdi;Ganganboina, Akhilesh Babu;Park, Enoch Y.
在目前的生物传感器中，信号的产生仅限于在反应室中检测单个病毒。需要一种自适应战略，以便能够识别多个病毒以进行诊断和监测。在这项工作中，根据目标病毒的不同，部署了一个纳米载体来将特定的信号放大引入生物传感器。该纳米载体是利用顺序纳米沉淀法制备的pH敏感的聚合物纳米颗粒载体(PNLN)设计的。研究了酚酞(PP)和胸腺素(TP)在三种不同溶剂体系中的纳米沉淀行为，结果表明PNLN在二甲基甲酰胺(DMF)/二甲基亚砜(DMSO)/乙醇中的载色量高达59.75%，这归因于发色原与聚合物的共沉淀程度。PP包裹的PNLN(PP@PNLN)和TP包裹的PNLN(TP@PNLN)分别与针对目标病毒H1N1亚型(IV/A/H1N1)和H3N2型(IV/A/H3N2型)的抗体连接。在添加抗IV/A抗体偶联磁性纳米颗粒(MNPs)和磁分离后，富集PNLns/病毒/MNPs夹心结构在碱性溶液中处理。它显示了一个协同反应，在这个反应中，聚合物边界的降解和pH诱导的色原显色。该传感器对IV/A具有良好的检测性能，对IV/A/H1N1的PP@PNLns和对IV/A/H3N2的TP@PNLns的检测灵敏度分别为27.56和28.38 fgmL(-1)。一步法检测亚型流感病毒的变异系数为25.8%，回收率为93.6-110.6%。
In the current biosensor, the signal generation is limited to single virus detection in the reaction chamber. An adaptive strategy is required to enable the recognition of multiple viruses for diagnostics and surveillance. In this work, a nanocarrier is deployed to bring specific signal amplification into the biosensor, depending on the target viruses. The nanocarrier is designed using pH-sensitive polymeric nanoparticle-laden nanocarriers (PNLNs) prepared by sequential nanoprecipitation. The nanoprecipitation of two chromogens, phenolphthalein (PP) and thymolphthalein (TP), is investigated in three different solvent systems in which PNLNs demonstrate a high loading of the chromogen up to 59.75% in dimethylformamide (DMF)/dimethyl sulfoxide (DMSO)/ethanol attributing to the coprecipitation degree of the chromogens and the polymer. The PPencapsulated PNLNs (PP@PNLNs) and TP-encapsulated PNLNs (TP@PNLNs) are conjugated to antibodies specific to target viruses, influenza virus A subtype H1N1 (IV/A/H1N1) and H3N2 (IV/A/H3N2), respectively. After the addition of anti-IV/A antibody-conjugated magnetic nanoparticles (MNPs) and magnetic separation, the enriched PNLNs/virus/MNPs sandwich structure is treated in an alkaline solution. It demonstrates a synergy reaction in which the degradation of the polymeric boundary and the pH-induced colorimetric development of the chromogen occurred. The derivative binary biosensor shows feasible detection on IV/A with excellent specificities of PP@PNLNs on IV/A/ H1N1 and TP@PNLNs on IV/A/H3N2 with LODs of 27.56 and 28.38 fg mL(-1), respectively. It intrigues the distinguished analytical signal in human serum with a variance coefficient of 25.8% and a recovery of 93.6- 110.6% for one-step subtype influenza virus detection.