Noise and information transmission in promoters with multiple internal States.
Noise and information transmission in promoters with multiple internal States.
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DOI：
10.1016/j.bpj.2014.01.014
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发表时间：
2013-07
影响因子：
3.4
通讯作者：
Georg Rieckh;G. Tkačik
中科院分区：
文献类型：
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作者：
Georg Rieckh;G. Tkačik
基于过去十年对基因表达噪声的测量，人们习惯于用两态模型来思考基因调控，在双态模型中，基因的启动子可以随机地在ON和OFF状态之间切换。随着实验变得越来越精确，与两态模型的偏差开始可观察到，我们询问复杂的多态启动子的实验签名，以及这种额外复杂性的功能后果。具体来说，我们将基因表达中噪声的计算推广到由多状态状态转移图描述的启动子，系统地计算了这些复杂启动子的实验可达噪声特性，并利用信息论评估了复杂启动子结构的通道容量，并将其与双态模型提供的基线进行了比较。我们发现，向启动子添加内部状态通常会降低通道容量，但在某些情况下除外，我们详细分析了其中三种情况(协作性、双重角色调节、启动子循环)。
Based on the measurements of noise in gene expression performed during the past decade, it has become customary to think of gene regulation in terms of a two-state model, where the promoter of a gene can stochastically switch between an ON and an OFF state. As experiments are becoming increasingly precise and the deviations from the two-state model start to be observable, we ask about the experimental signatures of complex multistate promoters, as well as the functional consequences of this additional complexity. In detail, we i), extend the calculations for noise in gene expression to promoters described by state transition diagrams with multiple states, ii), systematically compute the experimentally accessible noise characteristics for these complex promoters, and iii), use information theory to evaluate the channel capacities of complex promoter architectures and compare them with the baseline provided by the two-state model. We find that adding internal states to the promoter generically decreases channel capacity, except in certain cases, three of which (cooperativity, dual-role regulation, promoter cycling) we analyze in detail.