Low-dose radiation induces renal SOD1 expression and activity in type 1 diabetic mice
Low-dose radiation induces renal SOD1 expression and activity in type 1 diabetic mice
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低剂量辐射诱导 1 型糖尿病小鼠肾脏 SOD1 表达和活性
DOI：
10.3109/09553002.2014.877174
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发表时间：
2014-03-01
影响因子：
2.6
通讯作者：
Lu, Xuemian
中科院分区：
文献类型：
--
作者：
Zhang, Chi;Xing, Xiao;Lu, Xuemian
目的：氧化应激在糖尿病肾病（DN）的发病中起重要作用。作为一种抗氧化剂，超氧化物歧化酶（SOD）-1缺乏会加剧，但补充SOD 1可预防糖尿病引起的肾损伤。以前，我们已经证明，重复暴露于低剂量辐射（LDR）在25毫戈瑞显着防止DN。这种预防是否与SOD 1的表达和活性有关仍不清楚。本研究旨在探讨不同LDR处理方法对糖尿病小鼠肾脏SOD 1表达和活性的影响。材料与方法：采用链脲佐菌素（STZ）诱导C57 BL/6 J小鼠1型糖尿病模型。糖尿病小鼠用全身X射线以25 mGy或75 mGy的单次剂量或25 mGy的三次剂量照射，然后在不同时间处死。结果：LDR对正常组和糖尿病组小鼠的体重和血糖水平均无明显影响。结论：单次或累积75 mGy LDR刺激肾脏SOD 1表达和活性可能是糖尿病肾病的预防机制之一。
Purpose: Oxidative stress plays a critical role in the pathogenesis of diabetic nephropathy (DN). As an antioxidant, superoxide dismutase (SOD)-1 deficiency exacerbates but SOD1 supplementation prevents diabetes-induced renal damage. Previously, we have demonstrated that repetitive exposure to low-dose radiation (LDR) at 25 mGy significantly prevents DN. Whether this prevention is related to SOD1 expression and activity remains unknown. The aim of the present study was to explore the effects of different methods of LDR treatment on SOD1 expression and activity in the kidneys of diabetic mice.Materials and methods: C57BL/6J mice were induced with type 1 diabetes using streptozotocin (STZ). Diabetic mice were irradiated with whole-body X-rays at either a single dose of 25 mGy or 75 mGy, or three doses of 25 mGy and then sacrificed at different times. Body weight, blood glucose level, and renal SOD1 expression and activity were measured.Results: LDR had no impact on the body weights or blood glucose levels of the mice in either the normal or diabetic groups. A single exposure of LDR at 25 mGy did not preserve renal SOD1 expression and activity in diabetic mice, but a single exposure of LDR at 75 mGy or three exposures of LDR at 25 mGy could preserve them.Conclusion: The stimulation of renal SOD1 expression and activity by a single or cumulative LDR of 75 mGy may be one of the preventive mechanisms of DN observed in the previous study.