Protective effects of p-coumaric acid against oxidant and hyperlipidemia-an in vitro and in vivo evaluation
Protective effects of p-coumaric acid against oxidant and hyperlipidemia-an in vitro and in vivo evaluation
复制标题
对香豆酸的抗氧化剂和高脂血症保护作用的体外和体内评价
DOI：
10.1016/j.biopha.2018.12.074
复制
发表时间：
2019-03-01
影响因子：
7.5
通讯作者：
Guan, Yifu
中科院分区：
文献类型：
--
作者：
Shen, Yingbin;Song, Xun;Guan, Yifu
膳食酚类是具有多种生理功能的抗氧化剂，对人体健康有益。本研究旨在通过四种体外方法研究对香豆酸（p-CA）的抗氧化活性、对PC12细胞氧化应激的保护作用以及对高脂饮食（HFD）小鼠模型的降血脂作用。在体外实验中，p-CA表现出中等的抗氧化活性。在50 μ g/mL时，p-CA的螯合活性最高，为52.22%。p-CA预处理可显著提高PC12细胞活力，抑制aaph诱导的细胞内ROS生成和LDH释放。与HFD组相比，P - ca （100 mg/kg BW）的降血脂作用与核因子-红细胞2相关因子（Nrf2）、谷胱甘肽过氧化物酶（Gpx）、超氧化物歧化酶（SOD-1）、血红素加氧酶（HO-1）和NAD(P)H醌脱氢酶1 （NQO-1）的表达分别提高2.0倍、3.8倍、1.6倍、1.72倍和1.5倍有关。此外，与HFD组相比，p-CA降低了总胆固醇和动脉粥样硬化指数水平，提高了血清过氧化氢酶（CAT）水平、总抗氧化能力（T-AOC）和谷胱甘肽过氧化物酶（GSH-Px）水平。p-CA还通过改善脂质过氧化作用促进小鼠高脂血症脂肪性肝炎的恢复。这些结果表明，p-CA是一种有效的抗氧化剂，具有治疗高脂血症症状的潜在疗效。
Dietary phenols are antioxidants with diverse physiological functions that are beneficial for human health. The objective of this research work was to investigate antioxidant activity of p-coumaric acid (p-CA) using four in vitro methods, the protective effects against oxidative stress in PC12 cells, and hypolipidemic effects on High fat-diet (HFD) mice model. The p-CA exhibited moderate antioxidant activity in the selected in vitro assay. The highest chelating activity of p-CA at 50 mu g/mL was found to be 52.22%. Pretreatment with p-CA significantly enhanced cell viability of PC12 cell and suppressed AAPH-induced intracellular ROS generation and AAPH-induced LDH release. The hypolipidemic effects of p-CA (100 mg/kg BW) was directly linked to the increased expression of nuclear factor erythroid 2-related factor (Nrf2) by 2.0-fold, Glutathione peroxidase (Gpx) by 3.8-fold, Superoxide dismutase (SOD-1) by 1.6-fold, Heme oxygenase (HO-1) by 1.72-fold and NAD(P)H Quinone Dehydrogenase 1 (NQO-1) by 1.5-fold compared with HFD group. In addition to these effects, p-CA decreased total cholesterol and atherosclerosis index levels, and increased catalase (CAT) level in serum, total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) levels in liver as compared HFD group. Administration of p-CA also promoted the recovery of hyperlipidemia steatohepatitis in mice by ameliorating lipid peroxidation. These results suggested that p-CA is a potent antioxidant with potential therapeutic efficacy for treating hyperlipidemia symptoms.