Alterations in responsiveness of noradrenergic neurons of the locus coeruleus in deoxycorticosterone acetate (DOCA)-salt hypertensive rats.
Alterations in responsiveness of noradrenergic neurons of the locus coeruleus in deoxycorticosterone acetate (DOCA)-salt hypertensive rats.
复制标题
醋酸去氧皮质酮（DOCA）盐高血压大鼠蓝斑去甲肾上腺素能神经元反应性的改变。
DOI：
10.1016/0006-8993(87)91414-4
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Hofbauer,KG
中科院分区：
文献类型：
--
作者：
Berecek,KH;Olpe,HR;Mah,SC;Hofbauer,KG
蓝斑可能在中枢血压调节中发挥作用，并可能在高血压的发病机制中发挥作用。与这一观点相一致的是，我们最近发现，与对照动物相比，醋酸脱氧皮质酮（DOCA）盐高血压大鼠在蓝斑刺激引起的血压升高更大。为了阐明导致蓝斑反应性改变的潜在机制，使用常规方法在 DOCA 高血压前期（DOCA 后 3 天）、DOCA 慢性高血压（DOCA 后 6-8 周）和对照大鼠中研究了蓝斑去甲肾上腺素能神经元对候选递质、肾上腺素和谷氨酸的敏感性。 微离子电渗疗法和单细胞记录技术。在 DOCA 治疗组和对照组大鼠中，离子电渗疗法应用的肾上腺素导致所有去甲肾上腺素能神经元的自发放电率出现电流依赖性下降。 DOCA治疗第3天和第6-8周时的大鼠蓝斑神经元对肾上腺素的敏感性低于对照大鼠，自发放电率的抑制幅度也较小。相比之下，谷氨酸的离子电渗应用导致所有去甲肾上腺素能蓝斑神经元的活性增加。然而，DOCA 和对照大鼠的神经元之间的谷氨酸敏感性差异很小。由于肾上腺素敏感性的变化不仅在慢性期而且在高血压前期也很明显，因此我们的研究结果表明蓝斑的肾上腺素能输入在 DOCA 高血压的发病机制中具有潜在作用。
Locus coeruleus may have a function in central blood pressure regulation and possibly in the pathogenesis of hypertension. In kepping with this notion, we have recently shown that deoxycorticosterone acetate (DOCA)-salt hypertensive rats demonstrate a greater increase in blood pressure induced by locus coeruleus stimulation than control animals. In an attempt to elucidate the underlying mechanisms leading to this alteration in responsiveness of the locus coeruleus, the sensitivity of noradrenergic neurons of the locus coeruleus to the transmitter candidates, epinephrine and glutamate, was investigated in DOCA-prehypertensive (3 days post-DOCA), DOCA chronic hypertensive (6–8 weeks post-DOCA) and control rats using conventional microiontophoretic and single cell recording techniques. Iontophoretically applied epinephrine produced a current-dependent decrease in spontaneous firing rate of all noradrenergic neurons in both DOCA-treated and control rats. Locus coeruleus neurons of DOCA-treated rats at 3 days and 6–8 weeks were less sensitive to epinephrine than those of control rats and themagnitude of the depression in spontaneous firing rate was less. By contrast, iontophoretic applications of glutamate produced an increase in activity of all noradrenergic locus coeruleus neurons. However, there was minimal difference in glutamate sensitivity between neurons of DOCA and control rats. Since the changes in epinephrine sensitivity are apparent not only in the chronic stage but also in the prehypertensive stage, our findings suggest a potential role of the adrenergic input to the locus coeruleus in the pathogenesis of DOCA-hypertension.