Simultaneous analysis of highly polar pharmaceutical adulterants in slimming products by hydrophilic interaction liquid chromatography
Simultaneous analysis of highly polar pharmaceutical adulterants in slimming products by hydrophilic interaction liquid chromatography
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亲水作用液相色谱法同时分析减肥产品中的高极性药物掺假物
DOI：
10.1080/10826076.2016.1231691
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
H. Obana
中科院分区：
文献类型：
--
作者：
A. Nakamura;T. Tagami;K. Kajimura;K. Yamasaki;Y. Sawabe;H. Obana
建立了一种亲水作用液相色谱法检测市售减肥产品中高极性药物掺假的方法。在XBridge™酰胺色谱柱（3.5 µm，4.6 × 250 mm）上，使用由乙腈/26.5 mM甲酸铵（含0.1%甲酸）（85：15）组成的移动的流动相，以0.8 mL/min的流速成功分离了5种掺杂物（苯丙醇胺、沙丁胺醇、苯乙双胍、丁氨蝶呤和二甲双胍）。根据这些化合物的最大吸收波长（λmax= 208、226和236 nm），确定了监测这些化合物洗脱的波长。对开发方法的专属性、线性、准确度、精密度和耐用性进行了验证。批间比较表明，亲水作用液相色谱法的性能上级传统的反相液相色谱法。因此，所提出的方法是有用的分析高极性药物掺杂减肥产品。
A chromatographic method utilizing hydrophilic interaction liquid chromatography was developed for the estimation of highly polar pharmaceutical adulterants in commercial slimming products. Five adulterants, phenylpropanolamine, salbutamol, phenformin, buformin, and metformin, were successfully separated on an XBridge™amide column (3.5 µm, 4.6 × 250 mm) using a mobile phase composed of acetonitrile/26.5 mM ammonium formate containing 0.1% formic acid (85:15) flowing at the rate of 0.8 mL/min. Wavelengths for monitoring the elution of these compounds were determined based on the wavelength of their absorption maxima (λmax= 208, 226, and 236 nm). The developed method was validated for specificity, linearity, accuracy, precision, and robustness. Interassay comparison showed that the performance of the hydrophilic interaction liquid chromatography-based method was superior to that of the traditional reversed-phase liquid chromatography-based method. Therefore, the proposed method is useful to analyze highly polar pharmaceutical adulterants in slimming products.