Microsatellites can be misleading:: An empirical and simulation study
Microsatellites can be misleading:: An empirical and simulation study
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DOI：
10.1111/j.0014-3820.2000.tb00573.x
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发表时间：
2000-08-01
期刊：
影响因子：
3.3
通讯作者：
Goudet, J
中科院分区：
文献类型：
--
作者：
Balloux, F;Brünner, H;Goudet, J
人们早就认识到，高度多态性的遗传标记可能导致在低迁移时低估种群之间的差异。具有极高突变率特征的微卫星基因座尤其可能受到影响。在这里，我们报道了在普通鼩鼱（Sorex araneus）的两个染色体种族之间的接触区遗传分化的估计，基于10常染色体微卫星，一个新发展的y染色体微卫星和线粒体DNA。这些结果与先前关于蛋白质和核型的数据进行了比较。基于F-和r统计的遗传分化估计，常染色体微卫星比所有其他遗传标记低得多。我们通过模拟表明，这种差异主要源于f统计量的微卫星标记的高突变率和r统计量的单步突变模型的偏差。性别连锁的遗传标记表明，所有种族间的基因交换都是由雌性介导的。缺乏雄性介导的基因流最有可能是雄性杂交不育的结果。
It has been long recognized that highly polymorphic genetic markers can lead to underestimation of divergence between populations when migration is low. Microsatellite loci, which are characterized by extremely high mutation rates, are particularly likely to be affected. Here, we report genetic differentiation estimates in a contact zone between two chromosome races of the common shrew (Sorex araneus), based on 10 autosomal microsatellites, a newly developed Y-chromosome microsatellite, and mitochondrial DNA. These results are compared to previous data on proteins and karyotypes. Estimates of genetic differentiation based on F- and R-statistics are much lower for autosomal microsatellites than for all other genetic markers. We show by simulations that this discrepancy stems mainly from the high mutation rate of microsatellite markers for F-statististics and from deviations from a single-step mutation model for R-statistics. The sex-linked genetic markers show that all gene exchange between races is mediated by females. The absence of male-mediated gene flow most likely results from male hybrid sterility.