In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ.
In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ.
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DOI：
10.3791/54517
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发表时间：
2016-09
期刊：
影响因子：
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通讯作者：
Tobias Ackels;Daniela R. Drose;M. Spehr
中科院分区：
文献类型：
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作者：
Tobias Ackels;Daniela R. Drose;M. Spehr
在大多数哺乳动物中，犁鼻器(VNO)是一种化学感觉结构，可以检测到异种和同种的社交线索。犁鼻感觉神经元(VSN)表达至少三个不同化学受体基因家族的G蛋白偶联受体(GPCR)，使其能够敏感和特异地检测化学感觉线索。这些家族包括V1r和V2r基因家族，以及可能的化学受体基因的甲酰肽受体相关序列(FPR-Rs)家族。为了了解犁鼻受体-配体相互作用和下游信号的生理学，鉴定每一类特定的vSNs所固有的生物物理特性是必不可少的。这里描述的生理学方法允许使用转基因小鼠品系(FPR-RS3-I-Venus)来识别和深入分析确定的感觉神经元群体。然而，这种方案的使用并不局限于这一特定的品系，因此可以很容易地扩展到其他转基因品系或野生动物。
In most mammals, the vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Vomeronasal sensory neurons (VSNs) express a specific type of G protein-coupled receptor (GPCR) from at least three different chemoreceptor gene families allowing sensitive and specific detection of chemosensory cues. These families comprise the V1r and V2r gene families as well as the formyl peptide receptor (FPR)-related sequence (Fpr-rs) family of putative chemoreceptor genes. In order to understand the physiology of vomeronasal receptor-ligand interactions and downstream signaling, it is essential to identify the biophysical properties inherent to each specific class of VSNs. The physiological approach described here allows identification and in-depth analysis of a defined population of sensory neurons using a transgenic mouse line (Fpr-rs3-i-Venus). The use of this protocol, however, is not restricted to this specific line and thus can easily be extended to other genetically modified lines or wild type animals.