Fabrication of Thermo-Responsive Molecular Layers from Self-Assembling Elastin-Like Oligopeptides Containing Cell-Binding Domain for Tissue Engineering
Fabrication of Thermo-Responsive Molecular Layers from Self-Assembling Elastin-Like Oligopeptides Containing Cell-Binding Domain for Tissue Engineering
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DOI：
10.3390/polym7010134
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发表时间：
2015
期刊：
影响因子：
5
通讯作者：
T. Koga;K. Nakamoto;Koji Odawara;T. Matsuoka;N. Higashi
中科院分区：
文献类型：
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作者：
T. Koga;K. Nakamoto;Koji Odawara;T. Matsuoka;N. Higashi
制备了含细胞结合表位（Arg-Gly-Asp-Ser序列）的新型热响应性弹性蛋白样寡肽：精氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）-弹性蛋白样肽（ELP）和RGDS-deg-ELP。利用圆二色谱、傅里叶变换红外光谱、原子力显微镜和水接触角等技术对寡聚体（ELP）在水中的构象及其在疏水表面的自组装行为进行了详细的分析。实验结果表明，两种寡聚体（oligo，ELP）均能在疏水表面自组装形成分子层，其热响应性构象由含水无规卷曲转变为脱水β-转角结构.有效的细胞粘附和铺展行为观察到这些自组装寡（ELP）层。此外，附着的细胞被发现被成功地回收作为细胞片的寡聚（ELP）层的温度诱导的解体。这一成果为构建新型寡肽基纳米表面设计智能人工细胞外基质提供了重要的思路。
Novel thermo-responsive elastin-like oligopeptides containing cell-binding epitope (Arg-Gly-Asp-Ser sequence); arginine-glycine-aspartic acid-serine (RGDS)-elastin-like peptides (ELP) and RGDS-deg-ELP; were newly prepared as building blocks of self-assembled molecular layer for artificial extra cellular matrix. A detailed analysis of the conformation of the oligo(ELP)s in water and their self-assembling behavior onto hydrophobic surfaces were performed by using circular dichroism, Fourier transform infrared spectroscopy (FTIR), atomic force microscopy and water contact angle measurements. The experimental results revealed that both oligo(ELP)s self-assembled onto hydrophobic surfaces and formed molecular layers based on their thermo-responsive conformational change from hydrous random coil to dehydrated β-turn structure. Effective cell adhesion and spreading behaviors were observed on these self-assembled oligo(ELP) layers. In addition, attached cells were found to be recovered successfully as a cell-sheet by temperature-induced disassembly of oligo(ELP) layer. This achievement provides an important insight to construct novel oligopeptide-based nano-surfaces for the design of smart artificial extra-cellular matrix.