Novel advances in shotgun lipidomics for biology and medicine.
Novel advances in shotgun lipidomics for biology and medicine.
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DOI：
10.1016/j.plipres.2015.12.002
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发表时间：
2016-01
影响因子：
13.6
通讯作者：
Han X
中科院分区：
文献类型：
--
作者：
Wang M;Wang C;Han RH;Han X
自2003年诞生以来，脂质组学领域取得了深刻的进展，并得到了迅速的扩展。这一以分析方法学为导向的脂类分析研究学科基于质谱学的策略主要分为三类：基于直接输注的鸟枪型脂类组学、基于液相色谱-质谱学的平台和基于基质辅助激光解吸/电离质谱学的方法(尤其是在想象组织或细胞中的脂类分布方面)。本文综述了鸟枪型脂质组学的研究进展。在简要介绍了它的基本原理之后，本文的主要材料涵盖了它的最新进展。这些包括脂类提取的新方法，用于鉴定和量化以前难以获得的脂类和分子种类(包括异构体)的新的鸟枪式脂组学策略，以及用于处理和解释脂组数据的新工具。本文还介绍了先进的鸟枪脂组学在生物和生物医学研究中的典型应用。我们认为，随着鸟枪型脂质组学的这些新进展，这种脂类分析方法将变得更加全面和高通量，从而极大地加速脂类组学领域的研究，以证实病理条件下异常的脂代谢、信号、转运和动态平衡及其支持的生化机制。
The field of lipidomics, as coined in 2003, has made profound advances and been rapidly expanded. The mass spectrometry-based strategies of this analytical methodology-oriented research discipline for lipid analysis are largely fallen into three categories: direct infusion-based shotgun lipidomics, liquid chromatography-mass spectrometry-based platforms, and matrix-assisted laser desorption/ionization mass spectrometry-based approaches (particularly in imagining lipid distribution in tissues or cells). This review focuses on shotgun lipidomics. After briefly introducing its fundamentals, the major materials of this article cover its recent advances. These include the novel methods of lipid extraction, novel shotgun lipidomics strategies for identification and quantification of previously hardly accessible lipid classes and molecular species including isomers, and novel tools for processing and interpretation of lipidomics data. Representative applications of advanced shotgun lipidomics for biological and biomedical research are also presented in this review. We believe that with these novel advances in shotgun lipidomics, this approach for lipid analysis should become more comprehensive and high throughput, thereby greatly accelerating the lipidomics field to substantiate the aberrant lipid metabolism, signaling, trafficking, and homeostasis under pathological conditions and their underpinning biochemical mechanisms.