Polymorphism in the gene encoding the apical membrane antigen-1 (AMA-1) of Plasmodium falciparum.: X.: Asembo Bay Cohort Project
Polymorphism in the gene encoding the apical membrane antigen-1 (AMA-1) of Plasmodium falciparum.: X.: Asembo Bay Cohort Project
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DOI：
10.1016/s0166-6851(01)00229-8
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发表时间：
2001-04-06
影响因子：
1.5
通讯作者：
Lal, AA
中科院分区：
文献类型：
--
作者：
Escalante, AA;Grebert, HM;Lal, AA
我们研究了来自肯尼亚西部的恶性疟原虫自然种群中编码顶膜抗原 1 (AMA-1) 的基因的遗传多样性，并将其与来自其他地理区域的寄生虫种群进行了比较。共有 28 个完整序列来自肯尼亚。泰国、印度。获得了委内瑞拉田间分离株。遗传多态性在基因中的分布并不均匀。这与早期研究报告的模式一致。来自肯尼亚的等位基因表现出 20% 和 30% 的多态性，比东南亚和委内瑞拉等位基因的多态性更高。分别。根据测序数据得出的基因谱系，没有发现等位基因家族的证据。我们发现证据支持寄生虫种群之间的基因流动有限。具体来说。东南亚和委内瑞拉隔离区之间；然而，没有等位基因可以与特定地理区域联系起来。这项研究表明，积极的自然选择是维持 AMA-I 遗传多样性的重要因素。我们没有发现确凿的证据表明基因内重组对于 AMA-I 等位基因多样性的产生很重要。该研究提供了有关 AMA-1 基因遗传多样性的信息，可用于疫苗开发和测试。以及评估恶性疟原虫遗传多样性的产生和维持所涉及的因素。 (C) 2001 年由 Elsevier Science B.V. 出版
We have investigated the genetic diversity of the gene encoding the apical membrane antigen-1 (AMA-1) in natural populations of Plasmodium falciparum from western Kenya and compared it with parasite populations from other geographic regions. A total of 28 complete sequences From Kenya. Thailand, India. and Venezuela field isolates were obtained. The genetic polymorphism is not evenly distributed across the gene. which is in agreement with the pattern reported in earlier studies. The alleles from Kenya exhibit 20 and 30%, more polymorphism than that found in Southeast Asia and Venezuelan alleles. respectively. Based on the gene genealogies derived from sequencing data, no evidence for allele families was found. We have found evidence supporting limited gene flow between the parasite populations. specifically. between the Southeast Asian and Venezuelan isolates; however, no alleles could be linked to a specific geographic region. This study reveals that positive natural selection is an important factor in the maintenance of genetic diversity for AMA-I. We did not find conclusive evidence indicating intragenic recombination is important in the generation of the AMA-I allelic diversity. The study provides information on the genetic diversity of the AMA-1 gene that would be useful in vaccine development and testing. as well as in assessing factors that are involved in the generation and maintenance of the genetic diversity in P. falciparium. (C) 2001 Published by Elsevier Science B.V.