Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin
Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin
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DOI：
10.1016/j.cellbi.2003.09.007
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发表时间：
2003-01-01
影响因子：
3.9
通讯作者：
An, LJ
中科院分区：
文献类型：
--
作者：
Jiang, B;Liu, JH;An, LJ
本实验研究了葛根素对过氧化氢诱导的PC 12细胞凋亡的影响。细胞暴露于0.5 mM H2 O2可能会导致显著的活力丧失和凋亡率增加。以Ac-DEVD-AMC为底物，检测c-Myc、Bcl-2和Bax的表达及caspase-3的活性，发现这些凋亡调节和效应蛋白的变化提示c-Myc的升高、Bcl-2：Bax蛋白比值的降低和caspase-3的激活在细胞凋亡中起关键作用。当细胞在0.5 mM H2 O2处理之前用葛根素处理时，观察到活力丧失和凋亡率降低。此外，c-Myc表达降低，Bcl-2：Bax比值升高。葛根素还降低了H2 O2诱导的caspase-3活化的升高。这些结果表明，葛根素可以保护神经元免受氧化应激。它可以通过调节抗凋亡蛋白和促凋亡蛋白，以及通过减弱H2 O2诱导的PC 12细胞中caspase-3的活化来阻断早期凋亡。(C)2003爱思唯尔有限公司。保留所有权利。
In this study, the effect of puerarin on hydrogen peroxide-induced apoptosis in PC12 cells was studied. Exposure of cells to 0.5 mM H2O2 may cause significant viability loss and apoptotic rate increase. When c-Myc, Bcl-2 and Bax expression and caspase-3 activity were measured, using Ac-DEVD-AMC as a substrate, the changes in these apoptosis regulatory and effector proteins suggested that the elevation of c-Myc, decrease in Bcl-2:Bax protein ratio, and caspase-3 activation all play a key role in apoptosis. When cells were treated with puerarin prior to 0.5 mM H2O2 treatment, a reduction in viability loss and apoptotic rate was seen. In addition, c-Myc expression decreased and Bcl-2:Bax ratio increased. Puerarin also reduced the H2O2-induced elevation of caspase-3 activation. These results suggest that puerarin can protect neurons against oxidative stress. It can block apoptosis in its early stages via the regulation of anti- and pro-apoptotic proteins, as well as by the attenuation of caspase-3 activation in H2O2-induced PC12 cells. (C) 2003 Elsevier Ltd. All rights reserved.