POST-HARVEST VARIATION IN CELLULASE, POLYGALACTURONASE, AND PECTINMETHYLESTERASE IN AVOCADO (PERSEA-AMERICANA MILL, CV FUERTE) FRUITS IN RELATION TO RESPIRATION AND ETHYLENE PRODUCTION
POST-HARVEST VARIATION IN CELLULASE, POLYGALACTURONASE, AND PECTINMETHYLESTERASE IN AVOCADO (PERSEA-AMERICANA MILL, CV FUERTE) FRUITS IN RELATION TO RESPIRATION AND ETHYLENE PRODUCTION
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DOI：
10.1104/pp.64.2.306
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发表时间：
1979-01-01
期刊：
影响因子：
7.4
通讯作者：
YOUNG, RE
中科院分区：
文献类型：
--
作者：
AWAD, M;YOUNG, RE
从收获之日到果实分解开始，测定了单个“Fuerte”鳄梨果实的纤维素酶、多聚半乳糖醛酸酶（PG）、果胶甲酯酶（PME）、呼吸作用和乙烯产量。 PME 从采摘时的最大值下降到更年期早期的低水平。 PG活性在跃变前期未检测到，在跃变过程中增加，并在跃变后继续增加，达到果实达到可食用软质阶段时的三倍水平。跃变前果实中纤维素酶活性较低，随着呼吸作用的增强，纤维素酶活性开始升高，达到可食软化阶段的两倍。在检测到 PG 活性前 3 天，纤维素酶活性开始增加。呼吸作用和纤维素酶活性增加约 1.5 天后乙烯产量增加。这些结果表明细胞壁解聚酶的快速增加与呼吸和乙烯产生的增加之间存在密切关系，并重新将注意力集中在细胞壁和相关质膜在果实成熟早期事件中的作用。
Cellulase, polygalacturonase (PG), pectinmethylesterase (PME), respiration, and ethylene production were determined in single “Fuerte” avocado fruits from the day of harvest through the start of fruit breakdown. PME declined from its maximum value at the time of picking to a low level early in the climacteric. PG activity was not detectable in the preclimacteric stage, increased during the climacteric, and continued to increase during the postclimacteric phase to a level three times greater than when the fruit reached the edible soft stage. Cellulase activity was low in the preclimacteric fruit, started to increase just as respiration increased, and reached a level two times greater than at the edible soft stage. Cellulase activity started to increase 3 days before PG activity could be detected. Increased production of ethylene followed the increase in respiration and cellulase activity by about 1.5 days. These results indicate that a close relation exists between the rapid increase in the cell wall-depolymerizing enzymes and the rise in respiration and ethylene production and refocused attention on the role of the cell wall and the associated plasma membrane in the early events of fruit ripening.