Validation of a high-sensitivity assay for C-reactive protein in human saliva
Validation of a high-sensitivity assay for C-reactive protein in human saliva
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DOI：
10.1016/j.bbi.2010.12.020
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发表时间：
2011-05-01
影响因子：
15.1
通讯作者：
Arseneault, L.
中科院分区：
文献类型：
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作者：
Ouellet-Morin, I.;Danese, A.;Arseneault, L.
本研究旨在验证唾液中C-反应蛋白（CRP）的高灵敏度检测方法，作为研究大型流行病学队列和年轻人炎症的替代介质。我们测量了61名（29.5%男性）健康成人志愿者唾液和血清中的CRP。我们发现唾液和血清中CRP的测量值之间存在中度至强相关性（r = 0.72，p <0.001）。与CRP的非甾体结构和高分子量一致，我们观察到低唾液-血清CRP比（1：1633.64）。此外，唾液CRP的二分指数，相当于临床相关的血清CRP临界值（3 mg/l），与已知的全身炎症相关性（IL-6、BMI和吸烟）相关。最后，我们表明，CRP在唾液中是稳定的，在室温下长达8小时后收集。我们的研究提供了初步的证据表明，无创性评估CRP在唾液中允许有效的预测血清CRP。因此，唾液CRP可以促进和促进流行病学研究中探索低度炎症相关性的研究，并使将心理神经免疫学研究扩展到儿科人群成为可能。(C)2011 Elsevier Inc. All rights reserved.
This study aimed to validate a high-sensitivity assay for C-reactive protein (CRP) in saliva as an alternative medium to study inflammation in large epidemiological cohorts and young people. We measured CRP in saliva and serum in 61 (29.5% males) healthy adult volunteers. We found a moderate-to-strong association between CRP measured in saliva and in serum (r = .72, p < .001). In agreement with the non-steroidal structure and the high molecular weight of CRP, we observed a low saliva-to-serum CRP ratio (1:1633.64). Furthermore, a dichotomous index of salivary CRP, equivalent to a clinically relevant serum CRP cut-off (3 mg/l), was associated to known correlates of systemic inflammation (IL-6, BMI and smoking). Finally, we showed that CRP in saliva is stable at room temperature up to 8 h after collection. Our study provides initial evidence suggesting that non-invasive assessment of CRP in saliva allows valid prediction of serum CRP. Salivary CRP may thus facilitate and promote research exploring the correlates of low-grade inflammation in epidemiological studies and makes it feasible to expand psychoneuroimmunology research to pediatric populations. (C) 2011 Elsevier Inc. All rights reserved.