Nitrogen Shielding of an Argon Plasma Jet and Its Effects on Human Immune Cells
Nitrogen Shielding of an Argon Plasma Jet and Its Effects on Human Immune Cells
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DOI：
10.1109/tps.2015.2393379
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发表时间：
2015-03-01
影响因子：
1.5
通讯作者：
Weltmann, Klaus-Dieter
中科院分区：
文献类型：
--
作者：
Bekeschus, Sander;Iseni, Sylvain;Weltmann, Klaus-Dieter
大气压等离子体广泛用于生物医学或临床应用的研究。由等离子体产生的活性氧和活性氮被认为在等离子体-细胞相互作用中具有重要意义。然而，不同的应用可能需要不同的等离子体性质。通过调节供应气体或周围环境来定制等离子体装置是改变反应性物质组成、真空紫外线发射或温度的合适方式。涉及氧气或湿度可用性的治疗方案可能导致液体中过氧化氢沉积增加，因此对细胞有毒。使用大气压氩等离子体射流，我们在治疗人类免疫细胞期间对其可见流出物施加氮气幕。帷幕使等离子体失去了分子氧。这排除了气相氧等离子体化学，并导致高能量可燃烧物的产生。平面激光诱导荧光光谱验证层流气体流动和周围空气的气幕完全消除。我们使用人免疫细胞来监测细胞毒性效应，因为它们与潜在的临床血浆应用高度相关，例如，治疗慢性伤口。与氮幕等离子体处理相比，空气幕等离子体处理导致显著更高的细胞毒性。清除过氧化氢废除细胞死亡，在这两个气幕条件。这表明高能可燃物或气体温度升高对细胞毒性的影响可忽略不计。最后，结果表明，过氧化氢的氧非依赖性的产生，指出了UV或RNS在等离子体介导的细胞毒性中的间接作用。
Atmospheric pressure plasmas are widely used in research for biomedical or clinical applications. Reactive oxygen species and reactive nitrogen species (RNS) produced by plasmas are thought to be of major significance in plasma-cell interactions. Different applications, however, may demand for different plasma properties. Tailoring plasma devices by modulating the supply gas or the surrounding is a suitable way to alter reactive species composition, vacuum ultra violet emission, or temperature. Treatment regimens involving availability of oxygen or humidity may lead to increased hydrogen peroxide deposition in liquids and thus will be toxic to cells. Using an atmospheric pressure argon plasma jet, we applied a nitrogen gas curtain to its visible effluent during treatment of human immune cells. The curtain deprived the plasma of molecular oxygen. This excluded gas-phase oxygen plasma chemistry and led to generation of highly energetic metastables. Planar laser-induced fluorescence spectroscopy verified laminar gas flow and complete elimination of surrounding air by the gas curtain. We used human immune cells to monitor cytotoxic effects as they are highly relevant in potential clinical plasma applications, e.g., treatment of chronic wounds. Air curtain plasma treatment led to significantly higher cytotoxicity compared with nitrogen curtain plasma treatment. Scavenging of hydrogen peroxide abrogated cell death in both gas curtain conditions. This indicated a negligible contribution of highly energetic metastables or increased gas temperature to cytotoxicity. Finally, the results suggested an oxygen-independent generation of hydrogen peroxide pointing to an indirect role of UV or RNS in plasma-mediated cytotoxicity.