Intraflagellar transport balances continuous turnover of outer doublet microtubules: implications for flagellar length control.
Intraflagellar transport balances continuous turnover of outer doublet microtubules: implications for flagellar length control.
复制标题
鞭毛内运输平衡外部双联微管的连续周转：对鞭毛长度控制的影响。
DOI：
10.1083/jcb.200106141
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发表时间：
2001-10-29
影响因子：
7.8
通讯作者：
Rosenbaum, J L
中科院分区：
文献类型：
--
作者：
Marshall, W F;Rosenbaum, J L
细胞生物学的一个中心问题是细胞如何决定其细胞器的大小。鞭毛长度控制是研究细胞器大小调节的一个方便的系统。鞭毛长度调节机制模型的提出受到了限制的假设，鞭毛是静态结构，一旦他们组装。然而，最近的研究表明，鞭毛是动态的，并不断翻转。我们已经确定，这种营业额发生在鞭毛尖端，和组装部分的营业额介导的鞭毛内运输（IFT）。阻断IFT抑制微管蛋白在鞭毛尖端的掺入并导致鞭毛再吸收。这些结果导致一个简单的稳态模型鞭毛长度调节的组装和拆卸的平衡，可以有效地调节鞭毛长度。
A central question in cell biology is how cells determine the size of their organelles. Flagellar length control is a convenient system for studying organelle size regulation. Mechanistic models proposed for flagellar length regulation have been constrained by the assumption that flagella are static structures once they are assembled. However, recent work has shown that flagella are dynamic and are constantly turning over. We have determined that this turnover occurs at the flagellar tips, and that the assembly portion of the turnover is mediated by intraflagellar transport (IFT). Blocking IFT inhibits the incorporation of tubulin at the flagellar tips and causes the flagella to resorb. These results lead to a simple steady-state model for flagellar length regulation by which a balance of assembly and disassembly can effectively regulate flagellar length.