CHOLINERGIC PROJECTIONS FROM THE BASAL FOREBRAIN TO THE FRONTAL-CORTEX - A COMBINED FLUORESCENT TRACER AND IMMUNOHISTOCHEMICAL ANALYSIS IN THE RAT
CHOLINERGIC PROJECTIONS FROM THE BASAL FOREBRAIN TO THE FRONTAL-CORTEX - A COMBINED FLUORESCENT TRACER AND IMMUNOHISTOCHEMICAL ANALYSIS IN THE RAT
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DOI：
10.1016/0304-3940(83)90285-9
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
BUTCHER, LL
中科院分区：
文献类型：
--
作者：
WOOLF, NJ;ECKENSTEIN, F;BUTCHER, LL
胆碱能投射从基底前脑额叶皮质的一些地区进行了研究，注入碘化丙啶（PI），荧光示踪剂，到6和10区，并在显微镜下评估PI和胆碱-O-乙酰转移酶（ChAT）的细胞共定位。对相同的脑切片进行乙酰胆碱酯酶（AChE，药物组织化学方案）和尼氏物质（甲酚紫染色）的额外处理。基底前脑神经元投射到额叶皮质主要发现在基底核，但其他人位于与无名质/外侧视前区，大细胞视前区，和袢。这些投射神经元很大（最大索马范围> 25 μ m），表现出ChAT样免疫反应性，在全身施用双-（1-甲基-乙基）氟磷酸酯后对AChE强烈染色，并且是高度嗜色的。
Cholinergic projections from the basal forebrain to some regions of the frontal cortex were studied by infusing propidium iodide (PI), a fluorescent tracer, into areas 6 and 10, and microscopically assessing the cellular co-localization of PI and immunohistochemically demonstrated choline-O-acetyltransferase (ChAT). The same brain sections were additionally processed for acetylcholinesterase (AChE, pharmacohistochemical regimen) and Nissl material (cresyl violet stain). Basal forebrain neurons projecting to the frontal cortex were found primarily in nucleus basalis, but others were located in association with the substantia innominata/lateral preoptic area, magnocellular preoptic area, and ansa lenticularis. These projection neurons were large (> 25 .mu.m in maximum soma extent), demonstrated ChAT-like immunoreactivity, stained intensely for AChE following systemic administration of bis-(1-methyl-ethyl)phosphorofluoridate, and were highly chromophilic.