Novel O‐superfamily Conotoxins Identified by cDNA Cloning From Three Vermivorous Conus Species
Novel O‐superfamily Conotoxins Identified by cDNA Cloning From Three Vermivorous Conus Species
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DOI：
10.1111/j.1747-0285.2006.00443.x
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发表时间：
2006-11
影响因子：
3
通讯作者：
Dongting Zhangsun;S. Luo;Yong Wu;Xiaopeng Zhu;Yuanyan Hu;Lei Xie
中科院分区：
文献类型：
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作者：
Dongting Zhangsun;S. Luo;Yong Wu;Xiaopeng Zhu;Yuanyan Hu;Lei Xie
芋螺毒素O-超家族包括几个具有不同药理作用靶点的亚家族，它们都是电压门控离子通道，广泛分布于不同的螺螺物种中。任何圆锥体物种的毒液成分都与其他物种的毒素成分截然不同。通过基因克隆的方法，从海南产的3种食虫圆锥虫中分离鉴定出7个新的O-超家族多肽。它们共享三个共同的信号序列和一个保守的半胱氨酸残基排列(C-C-CC-C-C)。对本研究中新发现的芋螺毒素和已知的来自其他螺属物种的同源O超家族序列进行了系统的系统发育分析。信号区氨基酸序列的差异性和百分同源性表明，本研究中所描述的新型芋螺毒素属于三大分支：MSGL、ME-QK和MKLT，每个分支都有自己的特征信号序列和半胱氨酸密码子保守性。与这项工作相关的是，人们注意到O-超家族芋螺毒素在食虫物种中没有得到很好的代表。这些新发现的食虫毒素的cDNA的阐明将有助于更好地了解基础研究和药物开发。
The O‐superfamily of conotoxins includes several subfamilies with different pharmacological targets, all of which are voltage‐gated ion channels and distributed widely in varied Conus species. The venom components from any Conus species are quite distinct from those of other species. Seven novel O‐superfamily peptides were identified by cDNA cloning from the three vermivorous Conus species of C. betulinus, C. lividus and C. caracteristicus native to Hainan. They share three common signal sequences, and a conserved arrangement of cysteine residues (C–C–CC–C–C). Phylogenetic analysis of newly found conotoxins in this study and known homologue O‐superfamily sequences from the other Conus species was performed systematically. Divergence and percentage identity of the amino acid sequences of the signal regions suggest that the novel conotoxins described in this investigation belong to the three broad clades: MSGL, ME‐QK and MKLT, each of which has its own characteristic signature signal sequence and cysteine codon conservation. Relative to this work, it is noted that O‐superfamily conotoxins are not well represented from vermivorous species. The elucidated cDNAs of these newly found vermivorous toxins would facilitate a better understanding for basic research and drug discovery.