Tissue deformation modulates twist expression to determine anterior midgut differentiation in Drosophila embryos
Tissue deformation modulates twist expression to determine anterior midgut differentiation in Drosophila embryos
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DOI：
10.1016/j.devcel.2008.07.009
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发表时间：
2008-09-16
影响因子：
11.8
通讯作者：
Farge, Emmanuel
中科院分区：
文献类型：
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作者：
Desprat, Nicolas;Supatto, Willy;Farge, Emmanuel
与胚胎形态发生运动相关的机械变形被认为积极参与调节发育基因表达的信号级联。在这里，我们开发了一个适当的实验方法，以确定这种现象的存在和生理相关性。通过结合使用磁镊与体内激光消融，我们局部控制生理相关的野生型果蝇胚胎组织的变形。我们证明，由胚芽带延伸引起的变形上调气孔器原基中的Twist表达。我们发现，stomodeal压缩触发Src 42 A依赖的Armadillo/β-catenin，这是所需的扭曲机械诱导在stomodeum的核转位。最后，气孔特异性RNAi介导的Twist在压缩过程中的沉默损害了中肠细胞的分化，导致幼虫死亡。这些实验表明，在造口细胞中机械诱导的Twist上调对于随后的中肠分化是必要的。
Mechanical deformations associated with embryonic morphogenetic movements have been suggested to actively participate in the signaling cascades regulating developmental gene expression. Here we develop an appropriate experimental approach to ascertain the existence and the physiological relevance of this phenomenon. By combining the use of magnetic tweezers with in vivo laser ablation, we locally control physiologically relevant deformations in wild-type Drosophila embryonic tissues. We demonstrate that the deformations caused by germ band extension upregulate Twist expression in the stomodeal primordium. We find that stomodeal compression triggers Src42A-dependent nuclear translocation of Armadillo/beta-catenin, which is required for Twist mechanical induction in the stomodeum. Finally, stomodeal-specific RNAi-mediated silencing of Twist during compression impairs the differentiation of midgut cells, resulting in larval lethality. These experiments show that mechanically induced Twist upregulation in stomodeal cells is necessary for subsequent midgut differentiation.