Effect of extracellular matrix proteins on in vitro testosterone production by rat Leydig cells
Effect of extracellular matrix proteins on in vitro testosterone production by rat Leydig cells
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DOI：
10.1002/mrd.10111
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发表时间：
2002-04-01
影响因子：
2.5
通讯作者：
Denduchis, B
中科院分区：
文献类型：
--
作者：
Diaz, ES;Pellizzari, E;Denduchis, B
本研究的目的是检测细胞外基质（ECM）蛋白对大鼠睾丸间质细胞形态、粘附、整合素亚单位表达和睾酮分泌的影响。从成年大鼠分离的睾丸间质细胞培养板上未涂覆或涂有不同浓度的层粘连蛋白-1，纤连蛋白，或IV型胶原蛋白在存在或不存在的hCG为3或24 hr.A细胞粘附和α 3，α 5，和β 1整合素亚基表达的显着增加时，观察到细胞培养ECM蛋白，相比那些生长在未涂覆的板。在用ECM蛋白包被的玻璃盖玻片上培养24小时的间质细胞表现出具有长细胞突起的细长形状（铺展），而在未包被的板上培养的细胞显示出很少的细胞突起。与未涂覆的平板相比，当基础和hCG刺激的Leydig细胞在涂覆有IV型胶原（12和24 μ g/cm（2））的平板上培养3或24小时时，观察到睾酮产生显著减少。与未涂覆的平板相比，在用纤连蛋白（12和24 μ g/cm（2））涂覆的平板上培养的细胞中也观察到睾酮产生的显著但轻微的减少。层粘连蛋白-1没有修改睾酮的生产基础或hCG刺激的条件下。这些结果表明，ECM蛋白能够在体外调节Leydig细胞类固醇生成，（C）2002 Wiley-Liss，Inc.
The aim of this study was to detect the effect of extracellular matrix (ECM) proteins on rat Leydig cell shape, adhesion, expression of integrin subunits and testosterone production, in vitro. Leydig cells isolated from adult rats were cultured on plates uncoated or coated with different concentrations of laminin-1, fibronectin, or type IV collagen in the presence or absence of hCG for 3 or 24 hr. A significant increase of cell adhesion and of alpha3, alpha5, and beta1 integrin subunit expression was observed when cells were cultured on ECM proteins, compared to those grown on uncoated plates. Leydig cells cultured on glass coverslips coated with ECM proteins for 24 hr exhibited elongated shapes with long cell processes (spreading), while cells cultured on uncoated plates showed few cell processes. A significant decrease in testosterone production was observed when basal and hCG-stimulated Leydig cells were cultured for 3 or 24 hr on plates coated with type IV collagen (12 and 24 mug/cm(2)) compared to uncoated plates. A significant though a slighter decrease in testosterone production was also observed in cells cultured on plates coated with fibronectin (12 and 24 mug/cm(2)), compared to uncoated plates. Laminin-1 did not modify testosterone production under basal or hCG stimulated conditions. These results suggest that ECM proteins are able to modulate Leydig cell steroidogenesis, in vitro, (C) 2002 Wiley-Liss, Inc.