The ultrastructure of type I collagen at nanoscale: large or small D-spacing distribution?
The ultrastructure of type I collagen at nanoscale: large or small D-spacing distribution?
复制标题
DOI：
10.1039/c4nr01268b
复制
发表时间：
2014-06
期刊：
影响因子：
6.7
通讯作者：
H. Su;Li-Yuan Ran;Zhi-Hua Chen;Qi-Long Qin;Mei Shi;Xiao-yan Song;Xiulan Chen;Yu‐Zhong Zhang;B. Xie
中科院分区：
文献类型：
--
作者：
H. Su;Li-Yuan Ran;Zhi-Hua Chen;Qi-Long Qin;Mei Shi;Xiao-yan Song;Xiulan Chen;Yu‐Zhong Zhang;B. Xie
d -间距是I型胶原原纤维最重要的地形特征，对我们了解胶原的结构和功能具有重要意义。传统上，I型胶原纤维的d -间距显示为67 nm的奇异值，但最近的研究表明，当原子力显微镜测量d -间距值时，d -间距值具有较大的分布，可达10 nm。我们发现这种大的d -间距分布主要是由于测量过程中的图像漂移造成的。注意，在单个I型胶原纤维中，d -间距是均匀的。我们的统计分析表明，I型胶原原纤维的D-spacing值仅在67 nm附近呈2.5 nm的窄分布。此外，胶原原纤维的d -间距值不仅在单个原纤维束内几乎相同，而且在不同的原纤维束中也几乎相同。胶原蛋白D-spacing值的测定可以为胶原相关疾病、胶原分子模型的构建、胶原纤维形成等研究领域提供重要的结构信息。
D-Spacing is the most significant topographic feature of type I collagen fibril, and it is important for our understanding of the structure and function in collagens. Traditionally, the D-spacing of type I collagen fibril was shown to have a singular value of 67 nm, but recent works indicated that the D-spacing values have a large distribution of up to 10 nm when measured by atomic force microscopy. We found that this large distribution of D-spacing values mainly resulted from image drift during measurement. Note that the D-spacing was homogeneous in a single type I collagen fibril. Our statistical analysis indicated that the D-spacing values of type I collagen fibrils exhibited only a narrow distribution of 2.5 nm around the value of 67 nm. In addition, the D-spacing values of the collagen fibrils were nearly identical not only within a single fibril bundle, but also in different fibril bundles. The measurement of the D-spacing values of collagen may provide important structural information in many research areas such as collagen related diseases, construction of molecular model of collagen, and collagen fibrogenesis.