Label-free assessment of carotid artery biochemical composition using fiber-based fluorescence lifetime imaging
Label-free assessment of carotid artery biochemical composition using fiber-based fluorescence lifetime imaging
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DOI：
10.1364/boe.9.004064
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发表时间：
2018-09-01
影响因子：
3.4
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
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作者：
Alfonso-Garcia, Alba;Haudenschild, Anne K.;Marcu, Laura
需要能够监测生化成分变化并提供血管组织成熟的基准指标的新型诊断工具来创建功能性组织替代物。我们研究了基于纤维的，无标记的多光谱荧光寿命成像（FLIM）的能力，以量化的解剖变异的生化成分的天然颈动脉和验证这些结果对生化检测。在光谱带415-455 nm中的FLIM衍生参数与组织胶原蛋白含量（R-2 = 0.64）和细胞数量（R-2 = 0.61）相关，并且在光谱带465-553 nm中与弹性蛋白含量（R-2 = 0.89）强烈相关。这些结果表明，FLIM具有很大的潜力，用于评估血管组织的成熟和功能特性的基础上组织自体荧光。(C)根据OSA开放获取出版协议的条款，2018年美国光学学会
Novel diagnostic tools with the ability to monitor variations in biochemical composition and provide benchmark indicators of vascular tissue maturation are needed to create functional tissue replacements. We investigated the ability of fiber-based, label-free multispectral fluorescent lifetime imaging (FLIm) to quantify the anatomical variations in biochemical composition of native carotid arteries and validated these results against biochemical assays. FLIm-derived parameters in spectral band 415-455 nm correlated with tissue collagen content (R-2 = 0.64) and cell number (R-2 = 0.61) and in spectral band 465-553 nm strongly correlated with elastin content (R-2 = 0.89). These results suggest that FLIm holds great potential for assessing vascular tissue maturation and functional properties based on tissue autofluorescence. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement