Production of IL-27 and other IL-12 family cytokines by microglia and their subpopulations
Production of IL-27 and other IL-12 family cytokines by microglia and their subpopulations
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DOI：
10.1016/j.brainres.2005.01.100
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发表时间：
2005-04-08
期刊：
影响因子：
2.9
通讯作者：
Suzumura, A
中科院分区：
文献类型：
--
作者：
Sonobe, Y;Yawata, I;Suzumura, A
采用RT-PCR、实时荧光定量RT-PCR和Western blot分析检测小鼠小胶质细胞产生IL-27和其他IL-12家族细胞因子。我们首次表明，小鼠小胶质细胞产生IL-27响应脂多糖（LPS）和/或干扰素-γ。原代小胶质细胞，但不是它们的细胞系，也在上述刺激后诱导IL-12和IL-23。因此，小胶质细胞可能通过在脑中产生IL-12家族细胞因子而在启动Th 1应答中起关键作用。(c)2005 Elsevier B. V.保留所有权利。
Production of IL-27 and other IL-12 family cytokines by murine microglia were examined using RT-PCR, real-time RT-PCR and Western blot analysis. We show for the first time that murine microglia produce IL-27 in response to lipopolysaccharide (LPS) and/or interferon-gamma. Primary microglia, but not their cell lines, also induce IL-12 and IL-23 upon above stimulation. Therefore, microglia may play a critical role initiating Th1 responses via producing IL-12 family cytokines in the brain. (c) 2005 Elsevier B.V. All rights reserved.