Association of blood clotting factors I and VII with phospholipid monolayers at the air-water interface
Association of blood clotting factors I and VII with phospholipid monolayers at the air-water interface
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DOI：
10.1006/jcis.1999.6280
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发表时间：
1999-07-15
影响因子：
9.9
通讯作者：
Tran, N
中科院分区：
文献类型：
--
作者：
Serfis, AB;Katzenberger, R;Tran, N
吸附在空气-水界面上的磷脂单分子膜是研究磷脂与凝血因子I和凝血因子VII相互作用的有用模型膜。因子I是一种非膜结合蛋白，被发现在低脂压下可穿透二硬脂酰磷脂酰胆碱(DSPC)和二棕榈酰磷脂酰胆碱(DPPC)单分子膜。在高脂压力下，蛋白质被挤出界面。因子I磷脂单分子膜的渗透与碳氢链长度无关，而渗透率随电解质在亚相中的增加而最大，因子VII是一种膜结合蛋白，只有当电解质添加到亚相中时才能穿透DPPC单分子膜，无论亚相中电解质的添加如何，因子VII都能穿透DPPC单分子膜，其与DPPC膜的相互作用归因于蛋白质-脂类疏水相互作用，(C)1999学术出版社。
Phospholipid monolayers adsorbed at the air-water interface are useful model membranes and have been employed to study the interactions between phospholipids and blood clotting factors I and VII. Factor I is a non-membrane-binding protein and was found to penetrate both distearoylphosphatidylcholine (DSPC) and dipalmitoylphosphatidylcholine (DPPC) monolayers at low lipid pressures. At high lipid pressures, the protein was crowded out of the interface. Factor I penetration of phospholipid monolayers was independent of hydrocarbon chain length, while penetration was maximized with electrolytes in the subphase, Factor VII is a membrane binding protein and was found to penetrate a DPPC monolayer only when electrolytes were added to the subphase, Factor VII penetrated DSPC monolayers regardless of electrolyte addition in the subphase, and its interactions with DSPC films are attributed to protein-lipid hydrophobic interactions, (C) 1999 Academic Press.