A Hairpin Electrochemical Aptasensor for Sensitive and Specific Detection of Thrombin Based on Homogenous Target Recognition
A Hairpin Electrochemical Aptasensor for Sensitive and Specific Detection of Thrombin Based on Homogenous Target Recognition
复制标题
基于同质目标识别的发夹电化学适体传感器灵敏特异性检测凝血酶
DOI：
10.1002/elan.201200506
复制
发表时间：
2013-05-01
期刊：
影响因子：
3
通讯作者：
Yi, Gang
中科院分区：
文献类型：
--
作者：
Fu, Hu;Ge, Chuang;Yi, Gang
采用均相识别技术和纳米金粒子（AuNPs）扩增技术，研制了一种灵敏特异检测凝血酶的电化学配体传感器。以链霉亲和素碱性磷酸酶作为报告分子。与监测氧化还原分子与电极表面距离的传统发夹式适配体传感器相比，该传感器成功克服了距离的限制，通过同质识别提高了适配体发夹的稳定性和高亲和力，有效地提高了传感器的灵敏度和选择性。此外，aunp被用于增加电极的有效面积和电导率，从而提高了适体传感器的灵敏度。因此，所设计的凝血酶检测传感器在缓冲液中检测限为0.52 pM，在血清中检测限为6.9 pM。
An electrochemical aptasensor was developed for sensitive and specific detection of thrombin by combining homogenous recognition strategy and gold nanoparticles (AuNPs) amplification. Streptavidin-alkaline phosphatase was used as reporter molecule. Compared with the traditional hairpin aptasensor monitoring the distance of the redox molecule from the electrode surface, the proposed aptasensor successfully overcome the limitations of distance and improved the stability and high affinity of the aptamer hairpin through homogenous recognition, which enhanced the sensitivity and selectivity of the sensors effectively. Additionally, AuNPs were employed to increase the active area and conductivity of the electrode, thus, improving the sensitivity of the aptasensor. As a result, the designed thrombin detection sensor obtained a lower detection limit of 0.52 pM in buffer and 6.9 pM in blood serum.