EFFECTS OF 2-DEOXY-D-GLUCOSE ON HERPES-SIMPLEX VIRUS REPLICATION
EFFECTS OF 2-DEOXY-D-GLUCOSE ON HERPES-SIMPLEX VIRUS REPLICATION
复制标题
DOI：
10.1016/0042-6822(73)90340-1
复制
发表时间：
1973-01-01
期刊：
影响因子：
3.7
通讯作者：
BENYESHM.M
中科院分区：
文献类型：
--
作者：
COURTNEY, RJ;STEINER, SM;BENYESHM.M
研究了在不同浓度的2-脱氧-d-葡萄糖存在下培养的BSC 1细胞中单纯疱疹病毒（HSV）的复制。在所有测试浓度下，感染性病毒的产量比物理颗粒的产量受到更大程度的抑制，而被包膜的总颗粒的百分比保持恒定。在SDS-聚丙烯酰胺凝胶（SDS-PAGE）上对HSV诱导的糖蛋白的分析表明，随着2-脱氧-d-葡萄糖浓度的增加，病毒糖蛋白的表观分子量降低。对病毒诱导的蛋白质的SDS-PAGE分析表明，除了仅在含有较高浓度的2-脱氧-d-葡萄糖的HSV感染的培养物中可检测到的另外的组分之外，没有显著的改变。初步的SDS-PAGE分析表明，2-脱氧-d-葡萄糖被纳入HSV诱导的糖蛋白。
The replication of herpes simplex virus (HSV) was studied in BSC1 cells cultured in the presence of various concentrations of 2-deoxy-d-glucose. At all concentrations tested, the yield of infectious virus was inhibited to a greater degree than the yield of physical particles, while the percentage of the total particles which were enveloped remained constant. Analysis of HSV-induced glycoproteins on SDS-polyacrylamide gels (SDS-PAGE) indicated that as the concentration of 2-deoxy-d-glucose increased, there was a decrease in the apparent molecular weights of the viral glycoproteins. Analysis on SDS-PAGE of the viral-induced proteins indicated no significant alterations with the exception of an additional component detectable only in HSV-infected cultures containing the higher concentrations of 2-deoxy-d-glucose. Preliminary SDS-PAGE analysis indicated that 2-deoxy-d-glucose is incorporated into the HSV-induced glycoproteins.