Evolution of 2009 H1N1 influenza viruses during the pandemic correlates with increased viral pathogenicity and transmissibility in the ferret model
Evolution of 2009 H1N1 influenza viruses during the pandemic correlates with increased viral pathogenicity and transmissibility in the ferret model
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DOI：
10.1038/srep28583
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发表时间：
2016-06-24
影响因子：
4.6
通讯作者：
Gabriel, Guelsah
中科院分区：
文献类型：
--
作者：
Otte, Anna;Marriott, Anthony C.;Gabriel, Guelsah
越来越多的证据表明，2009年H1N1流感大流行病毒在大流行开始后发生了演变，在随后的几波中引发了严重的流行。然而，在大流行开始后，仍不清楚哪些病毒决定因素可能导致了疾病的严重程度。本研究表明，2009年H1N1大流行病毒基因组中不同的突变在大流行宣布后发生的频率增加。其中，发现了病毒血凝素的一种突变，该突变增加了2009年H1N1大流行病毒与类人α 2,6链唾液酸的结合。此外，这些突变增加了病毒在呼吸道中的复制，并增加了雪貂之间的呼吸道飞沫传播。因此，我们的数据表明，2009年H1N1流感病毒在大流行爆发后发生了进化，产生了新的病毒变体，在哺乳动物模型中增强了病毒的复制适应性和呼吸道飞沫传播。这些发现可能有助于改进监测工作，以评估新出现的流感病毒的大流行风险。
There is increasing evidence that 2009 pandemic H1N1 influenza viruses have evolved after pandemic onset giving rise to severe epidemics in subsequent waves. However, it still remains unclear which viral determinants might have contributed to disease severity after pandemic initiation. Here, we show that distinct mutations in the 2009 pandemic H1N1 virus genome have occurred with increased frequency after pandemic declaration. Among those, a mutation in the viral hemagglutinin was identified that increases 2009 pandemic H1N1 virus binding to human-like alpha 2,6-linked sialic acids. Moreover, these mutations conferred increased viral replication in the respiratory tract and elevated respiratory droplet transmission between ferrets. Thus, our data show that 2009 H1N1 influenza viruses have evolved after pandemic onset giving rise to novel virus variants that enhance viral replicative fitness and respiratory droplet transmission in a mammalian animal model. These findings might help to improve surveillance efforts to assess the pandemic risk by emerging influenza viruses.