Similarity of proteins synthesized by isolated blastomeres of early sea urchin embryos.
Similarity of proteins synthesized by isolated blastomeres of early sea urchin embryos.
复制标题
早期海胆胚胎分离的卵裂球合成的蛋白质的相似性。
DOI：
10.1016/0012-1606(79)90129-5
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发表时间：
1979
影响因子：
2.7
通讯作者：
B. Brandhorst
中科院分区：
文献类型：
--
作者：
F. Tufaro;B. Brandhorst
16细胞的海胆胚胎有三种不同大小的卵裂球：小分球、中分球和大分球。每一类都已经限制了其发育命运，微粒体致力于形成初级间充质细胞。以高纯度分离三类卵裂球，并在[35S]甲硫氨酸中孵育直至下一次裂解。几乎所有的放射性蛋白质都被溶解，并根据O 'Farrell进行了二维电泳。在分辨的大约1000个斑点中，三个卵裂球之间没有质的差异。当胚胎标记之间的第一和第四卵裂球，然后分离，没有观察到蛋白质合成的质的差异。此外，未受精的卵子与16细胞胚胎相比几乎没有变化。因此，胚胎发育过程中的细胞决定并不伴随着胚胎内大量合成蛋白质分布的质的变化，实际上所有这些蛋白质都是由卵中存在的序列编码的。
The 16-cell sea urchin embryo has blastomeres of three distinct size classes: micromeres, mesomeres, and macromeres. Each class is already restricted in its developmental fate, micromeres being committed to formation of primary mesenchyme cells. The three classes of blastomeres were isolated in high purity and incubated in [35S]methionine until the next cleavage. Nearly all the radioactive protein was solubilized and subjected to two-dimensional electrophoresis according to O'Farrell. Of approximately 1000 spots resolved, there are no qualitative differences among the three blastomeres. When embryos were labeled between the first and fourth cleavages and blastomeres then isolated, no qualitative differences in protein synthesis were observed. Moreover, there are very few changes when unfertilized eggs are compared to 16-cell embryos. Thus cellular determination during embryonic development is not accompanied by qualitative changes in the distribution within the embryo of abundantly synthesized proteins, virtually all of which are coded for by sequences present in the egg.