Methoods of Dendrobium Rust Detection and Analysis on the Genetic Structure of Dendrobium Rust Populations
Methoods of Dendrobium Rust Detection and Analysis on the Genetic Structure of Dendrobium Rust Populations
复制标题
石斛锈病检测方法及石斛锈病种群遗传结构分析
DOI：
10.17957/ijab/15.0971
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发表时间：
2019
影响因子：
--
通讯作者：
Liu Lin
中科院分区：
文献类型：
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作者：
Pu Shixian;Yu Hang;Li Guilin;Yang Jing;Li Zesheng;Bai Yanbing;Zhang Cui;Wang Na;Li Chengyun;Liu Lin
石斛属植物是我国一种珍稀药材，具有很高的药用价值。随着栽培年限的延长，铁皮石斛锈病已成为影响和制约其安全生产的主要生物学因素之一，但目前对铁皮石斛锈病的研究较少。本研究采集了红河、德宏铁皮石斛、无叶石斛、铁皮石斛和齿瓣石斛4个主要产区的150份锈病菌样品，进行了症状观察、病原鉴定和ITS序列分析。分别属于担子菌纲、冬孢纲、锈菌目、鞘孢菌科和鞘孢菌属。物种将在未来得到确认。此外，还利用ISSR技术对不同主产区铁皮石斛种群结构和同一地区不同种铁皮石斛种群结构进行了分析利用ISSR分子标记技术对红河、德宏两地石斛锈病病原菌进行了研究，结果表明，引起石斛锈病的枝孢菌主要是区域性聚集，红河和德宏两地的锈菌有少量聚集，引起石斛锈病的不同品种的枝孢菌单独聚集，表明石斛锈菌已呈现出较强的专化性。
Dendrobium, as a rare and precious medicinal material in China, has high medicinal value. With the extension of the cultivating years, one of the main biological factors to affect and restrict its safe production is Dendrobium rust, on which there are very few studies involved currently. Our research has collected a total of 150 rust fungi samples bred from Dendrobium candidum, Dendrobium aphyllum, Dendrobium crystallinum and Dendrobium devonianum from its main producing areas (Honghe and Dehong), and had performed the symptoms observation, pathogenetic identification and ITS sequence analysis, with the results indicated that samples of the all rust fungi of Dendrobium spp. were belonged to Basidiomycetes, Teliomycetes, Uredinales, Coleosporaceae, and Coleosporium. The species will be confirmed in the future. In addition, we had analyzed on the Dendrobium candidum population structure in different main producing areas and different species of Dendrobium in the same area by taking advantage of ISSR (Inter-simple sequence repeated, ISSR) molecular markers, with the results showed that the Cladosporium causing the Dendrobium rust in Honghe and Dehong was mainly aggregated regionally, and a small amount of rusts in Honghe was found to be aggregated with that in Dehong, and the Cladosporium bred from different varieties of Dendrobium causing the Dendrobium rust was aggregated individually, which indicated that the rust fungi of Dendrobium had presented a stronger specialization.