MECHANISM OF PHYTOHEMAGGLUTININ (PHA) ACTION .5. PHA COMPARED WITH CONCANAVALIN A(CON A)
MECHANISM OF PHYTOHEMAGGLUTININ (PHA) ACTION .5. PHA COMPARED WITH CONCANAVALIN A(CON A)
复制标题
DOI：
10.1016/0014-4827(72)90176-0
复制
发表时间：
1972-01-01
影响因子：
3.7
通讯作者：
LINDAHLK.K
中科院分区：
文献类型：
--
作者：
LINDAHLK.K
本文比较了PHA和Con A在短期人淋巴细胞培养中的刺激特性。PHA比Con A更强地与细胞结合。PHA的量比Con A的量少得多，足以达到最大刺激。在PHA培养物中，胸苷掺入的峰值通常提前1天出现。培养19 h后用α-甲基甘露糖苷去除Con A可降低有丝分裂活性。淋巴细胞暴露于Con A 8小时后，如果再用PHA或ALG刺激，则胸苷掺入量达到最大，尽管两种刺激都不足以引起DNA合成，但还不可能证明EDTA对Con A的促有丝分裂特性有任何明确的影响，尽管与淋巴细胞的结合似乎受到损害。然而，PHA在EDTA存在下根本不能与淋巴细胞结合。在生理盐水中酸化和透析的PHA不能诱导淋巴细胞中的胸苷掺入，尽管它可能与细胞结合。这表明，需要多个结合位点上的诱导分子，以实现有丝分裂诱导。
The stimulatory properties of PHA and Con A in short-term human lymphocyte cultures have been compared. PHA binds more strongly to the cells than does Con A. Much smaller quantities of PHA than of Con A suffice for maximal stimulation. The peak thymidine incorporation generally occurs 1 day earlier in PHA cultures. Removal of Con A with α-methylmannoside after up to 19 h in culture reduces the mitotic activity. Lymphocytes exposed to Con A for 8 h attain maximal thymidine incorporation if restimulated later on with PHA or ALG although neither stimulus equally timed is sufficient to cause DNA synthesis on its own.It has not been possible to demonstrate any definite effect of EDTA on the mitogenic properties of Con A although the binding to lymphocytes seems to be impaired. However, PHA cannot bind at all to lymphocytes in the presence of EDTA. Acidified and dialysed PHA in saline cannot induce thymidine incorporation in lymphocytes although it may be bound to the cells. It is suggested that multiple binding sites are required on the inducer molecule to achieve mitotic induction.