Esophageal epithelial cell interaction with synthetic and natural scaffolds for tissue engineering
Esophageal epithelial cell interaction with synthetic and natural scaffolds for tissue engineering
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DOI：
10.1016/j.biomaterials.2005.04.010
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发表时间：
2005-11-01
期刊：
影响因子：
14
通讯作者：
Giachelli, CM
中科院分区：
文献类型：
--
作者：
Beckstead, BL;Pan, S;Giachelli, CM
作为迈向组织工程化食管的第一步，分离大鼠食管上皮细胞（REEC），并表征上皮身份，粘附蛋白偏好，以及与天然和合成支架的体外相互作用。支架由AlloDerm（LifeCell Corporation，Branchburg，NJ）、聚（L-乳酸）（PLLA）、聚（乳酸-共-乙醇酸）（75：25）（PLGA 75）、聚（乳酸-共-乙醇酸）（50：50）（PLGA 50）和聚己内酯/聚（L-乳酸）（50：50）（PCL/PLLA）组成。各种因素，包括钙浓度，支架成分，和孔径进行了评估，他们对上皮细胞的生长和分化的影响。到第18天，在高Ca（+）（+）（1.5 mm）条件下培养的接种在AlloDerm上的角质形成细胞显示增殖的基底细胞层、上皮分层（5-6层）和厚的角蛋白层。合成支架（PLGA、PLLA、PCL/PLLA）还显示出完全的表面覆盖、增殖基底细胞的区域以及分层（2-3层）和角化的证据。然而，合成支架的高度多孔性限制了连续上皮层的形成，并导致缺乏整体空间定义的分化。总之，大鼠食管上皮细胞被成功分离和表征，与合成支架相比，接种在AlloDerm上的细胞显示出上级上皮组织和分层。合成支架的表面性质和孔径的修改可能是必要的，以模仿天然支架上的上皮行为。(c)2005爱思唯尔有限公司保留所有权利。
As an initial step towards a tissue-engineered esophagus, rat esophageal epithelial cells (REEC) were isolated and characterized for epithelial identity, adhesion protein preference, and in vitro interaction with natural and synthetic scaffolds. The scaffolds consisted of AlloDerm (LifeCell Corporation, Branchburg, NJ), poly(L-lactic acid) (PLLA), poly(lactic-co-glycolic) acid (75:25) (PLGA75), poly(lactic-co-glycolic) acid (50:50) (PLGA50), and polycaprolactone/poly(L-lactic acid) (50:50) (PCL/PLLA). Various factors-including calcium concentration, scaffold composition, and pore size-were evaluated for their influence on epithelial growth and differentiation. By day 18, keratinocytes seeded on AlloDerm cultured under high Ca (+) (+) (1.5 mm) conditions showed a proliferating basal cell layer, epithelial stratification (5-6 layers) and a thick keratin layer. The synthetic scaffolds (PLGA, PLLA, PCL/PLLA) also showed complete surface coverage, regions of proliferating basal cells, and evidence of stratification (2-3 layers) and keratinization. The highly porous nature of the synthetic scaffolds, however, limited the formation of a continuous epithelial layer and resulted in a lack of overall spatially-defined differentiation. In conclusion, rat esophageal epithelial cells were successfully isolated and characterized, with cells seeded on AlloDerm showing superior epithelial organization and stratification compared to synthetic scaffolds. Modification of the synthetic scaffold's surface properties and pore size may be necessary to mimic epithelial behavior on natural scaffolds. (c) 2005 Elsevier Ltd. All rights reserved.