DELAYED APPEARANCE OF LETHAL AND SPECIFIC GENE-MUTATIONS IN IRRADIATED MAMMALIAN-CELLS
DELAYED APPEARANCE OF LETHAL AND SPECIFIC GENE-MUTATIONS IN IRRADIATED MAMMALIAN-CELLS
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DOI：
10.1016/0360-3016(90)90354-m
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发表时间：
1990-12-01
影响因子：
7
通讯作者：
VETROVS, H
中科院分区：
文献类型：
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作者：
LITTLE, JB;GORGOJO, L;VETROVS, H
我们研究了辐射CHO和BALB/3 T3细胞的后代中致死性和6-硫代鸟嘌呤（hprt基因座）突变体的发生。 致死突变的表达，如通过子代细胞群体中降低的克隆效率所测量的，在X-辐射后检测到高达30个平均群体加倍。 初步证据表明，hprt基因座突变的表达也可能延迟至少6-7个群体时间。 这些结果表明，辐射可引起细胞遗传不稳定，导致自发突变率增加，并持续细胞分裂多代。 这些研究结果进行了讨论，在其可能的影响照射的肿瘤细胞群体在体内的反应。
We have examined the occurrence of lethal and 6-thioguanine (hprt locus) mutants among the progeny of irradiated CHO and BALB/3T3 cells. The expression of lethal mutations, as measured by a reduced cloning efficiency in the progeny cell population, was detected population, was detected up to 30 mean population doubles after x-radiation. Preliminary evidence indicates that the expression of mutations at the hprt locus may also be delayed for at least 6-7 population doublings. These results suggest that radiation can induce genetic instability in cells, resulting in an increased rate of spontaneous mutations which persists for many generations of cell division. These findings are discussed in terms of their possible influence on the response of irradiated tumor cell populations in vivo.