Characterization of a long-wavelength feature in the absorption and circular dichroism spectra of beta-nicotinamide adenine dinucleotide. Evidence for a charge transfer transition.
Characterization of a long-wavelength feature in the absorption and circular dichroism spectra of beta-nicotinamide adenine dinucleotide. Evidence for a charge transfer transition.
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β-烟酰胺腺嘌呤二核苷酸的吸收和圆二色性光谱中长波长特征的表征。
DOI：
10.1021/bi00603a028
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
R. Woody
中科院分区：
文献类型：
--
作者：
R. Reisbig;R. Woody
Richard R. Reisbig 和 Robert W. Woody* 摘要：ß-AD+ 的吸收光谱具有弱而宽的特征，延伸至远高于 300 nm 的波长。在圆二色性 (CD) 光谱中观察到相应的正带。 a-NAD+ 具有较强的长波长吸收带和 312 nm 处的负 CD 带，与 260 nm 主带符号相反。降低 pH 值、升高温度或添加有机溶剂会显着减弱这些长波长特征。 β-和 a-NAD+ 的酶裂解消除了长波长吸收和 CD 带。我们还研究了吡啶-3-醛腺嘌呤二核苷酸、3-乙酰基吡啶腺嘌呤二核苷酸、脱氨基-NAD+和NADP+。
Richard R. Reisbig and Robert W. Woody* abstract: The absorption spectrum of ß-AD+ has a weak, broad feature extending to wavelengths well above 300 nm. A corresponding positive band is observed in the circular dichroism (CD) spectrum. a-NAD+ has a somewhat stronger long-wavelength band in absorption and a negative CD band at 312 nm, opposite in sign to the main 260-nm band. These long-wavelength features are markedly diminished by lowering the pH, raising the temperature, or adding organic solvents. Enzymatic cleavage of both ß-and a-NAD+ abolishes the long-wavelength absorption and CD bands. We have also studied pyridine-3-aldehyde adenine dinucleotide, 3-acetylpyridine adenine dinucleotide, deamino-NAD+, and NADP+.