Ultrastructural and light microscopic evidence of apoptosis after middle cerebral artery occlusion in the rat.
Ultrastructural and light microscopic evidence of apoptosis after middle cerebral artery occlusion in the rat.
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发表时间：
1995-05
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通讯作者：
Y. Li;Victor G. Sharov;N. Jiang;C. Zaloga;H. Sabbah;M. Chopp
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作者：
Y. Li;Victor G. Sharov;N. Jiang;C. Zaloga;H. Sabbah;M. Chopp
8只大鼠在短暂性局灶性脑缺血(2小时)和再灌流(22小时)后，脑组织呈现细胞凋亡的超微结构和DNA片段化的光镜特征。同侧大脑半球DNA片段化细胞数(216+/-43个/切片)显著高于对侧、正常大鼠(n=4)和假手术(n=4)大鼠(对照组为0~3个阳性细胞/切片)。显示DNA片段化的细胞主要位于梗塞的内边界区域。显示DNA片段化的细胞提供了细胞凋亡的形态信息。细胞凋亡可能参与短暂性局灶性脑缺血后脑梗塞的发生发展。
Brains from eight rats subjected to transient focal ischemia (2 hours) and reperfusion (22 hours) revealed an ultrastructural appearance of apoptosis and light microscopic features of cells exhibiting DNA fragmentation. The number of cells exhibiting DNA fragmentation was significantly higher (P < 0.01) in the ipsilateral hemisphere (216 +/- 43 per section) than in the contralateral hemisphere and in normal (n = 4) and sham operated (n = 4) rats (0 to 3 positive cells per section in controls). Cells exhibiting DNA fragmentation were primarily located in the inner boundary zone to the infarct. Cells exhibiting DNA fragmentation provided morphological information of apoptosis. Apoptosis may contribute to the development of infarct after transient focal cerebral ischemia.