The septins are required for the mitosis-specific activation of the Gin4 kinase.
The septins are required for the mitosis-specific activation of the Gin4 kinase.
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DOI：
10.1083/jcb.143.3.709
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发表时间：
1998-11-02
期刊：
影响因子：
--
通讯作者：
Kellogg D
中科院分区：
文献类型：
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作者：
Carroll CW;Altman R;Schieltz D;Yates JR;Kellogg D
在萌芽酵母中，一种名为Gin4的蛋白激酶在有丝分裂期间被特异性地激活，并在Clb2周期蛋白启动的途径中发挥作用，以控制芽的生长。我们已经使用遗传学和生物化学来确定在这一途径中与Gin4一起发挥功能的其他蛋白质，这两种方法都已经确定了Septin家族的成员。Septin功能的丧失会产生一种与Gin4功能丧失引起的表型非常相似的表型，并且在有丝分裂早期需要Septins来激活Gin4激酶的活性。此外，Septin突变体在短纺锤体阶段表现出较长的有丝分裂延迟，这与Septin在控制有丝分裂事件中的作用是一致的。Septin家族的成员直接与Gin4结合，表明Gin4和Septins的功能必须在细胞内紧密联系。这些结果表明，芽殖酵母中的分隔素在Gin4激酶有丝分裂的特异性调节中起着不可或缺的作用，并且它们在有丝分裂的早期就发挥了作用。
In budding yeast, a protein kinase called Gin4 is specifically activated during mitosis and functions in a pathway initiated by the Clb2 cyclin to control bud growth. We have used genetics and biochemistry to identify additional proteins that function with Gin4 in this pathway, and both of these approaches have identified members of the septin family. Loss of septin function produces a phenotype that is very similar to the phenotype caused by loss of Gin4 function, and the septins are required early in mitosis to activate Gin4 kinase activity. Furthermore, septin mutants display a prolonged mitotic delay at the short spindle stage, consistent with a role for the septins in the control of mitotic events. Members of the septin family bind directly to Gin4, demonstrating that the functions of Gin4 and the septins must be closely linked within the cell. These results demonstrate that the septins in budding yeast play an integral role in the mitosis-specific regulation of the Gin4 kinase and that they carry out functions early in mitosis.