Gene expression atlas for human embryogenesis
Gene expression atlas for human embryogenesis
复制标题
人类胚胎发生基因表达图谱
DOI：
10.1096/fj.10-158782
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发表时间：
2010-09-01
期刊：
影响因子：
4.8
通讯作者：
Li, Wen-Xin
中科院分区：
文献类型：
--
作者：
Yi, Hong;Xue, Lu;Li, Wen-Xin
人类胚胎发育被认为涉及一系列复杂而协调的不同器官和组织的发育，这些器官和组织是由许多基因时空表达的变化所介导的。在这里，我们报告了在人类胚胎发生的第4-9周期间的全基因组表达分析，这是大多数器官发育的关键时期。大约有一半的人类基因表达，18.6%的表达基因在这一重要时期受到显著调控。我们进一步鉴定了>；5000个受调控的基因，其中大多数以前并不知道与动物发育有关。我们的研究填补了哺乳动物发育研究的一个重要空白，确定了参与这一关键但以前未被研究的时期的功能途径。我们的研究还发现，这里涉及的基因与早期胚胎发育过程中的基因不同，这些基因包括三组母体基因。此外，我们还发现，在特定的发育过程中，基因是协调调节的。这导致我们开发了一个易于搜索的整个基因表达谱集合的数据库，从而能够识别对特定发育过程/途径重要的新基因，并推断新基因的潜在功能。通过对这两个新基因的时空分析，用两个实例验证了数据库预测的有效性。这样的数据库将为人类发育和发病的分子分析提供非常有价值的资源。-易，H，薛，L，郭明祥，马杰，曾勇，Y.，王，W，蔡继勇，胡继勇，H-M，Shu，H-B，S，Y-B，Li，W-X。人类胚胎发生的基因表达图谱。FASE B J.24,3341-3350(2010)。Www.fasebj.org
Human embryogenesis is believed to involve an integrated set of complex yet coordinated development of different organs and tissues mediated by the changes in the spatiotemporal expression of many genes. Here, we report a genome-wide expression analysis during wk 4-9 of human embryogenesis, a critical period when most organs develop. About half of all human genes are expressed, and 18.6% of the expressed genes were significantly regulated during this important period. We further identified >5000 regulated genes, most of which previously were not known to be associated with animal development. Our study fills an important gap in mammalian developmental studies by identifying functional pathways involved in this critical but previously not studied period. Our study also revealed that the genes involved here are distinct from those during early embryogenesis, which include three groups of maternal genes. Furthermore, we discovered that genes in a given developmental process are regulated coordinately. This led us to develop an easily searchable database of this entire collection of gene expression profiles, allowing for the identification new genes important for a particular developmental process/pathway and deducing the potential function of a novel gene. The validity of the predictions from the database was demonstrated with two examples through spatiotemporal analyses of the two novel genes. Such a database should serve as a highly valuable resource for the molecular analysis of human development and pathogenesis.-Yi, H., Xue, L., Guo, M.-X. Ma, J., Zeng, Y., Wang, W., Cai, J.-Y. Hu, H.-M., Shu, H.-B. Shi, Y.-B., Li, W.-X. Gene expression atlas for human embryogenesis. FASEB J. 24, 3341-3350 (2010). www.fasebj.org