Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells.
Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells.
复制标题
完整 Novikoff 肝癌细胞中细胞角蛋白 p39 与 DNA 的关联。
DOI：
10.1073/pnas.81.2.419
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Hnilica,LS
中科院分区：
文献类型：
--
作者：
Ward,WS;Schmidt,WN;Schmidt,CA;Hnilica,LS
大鼠肿瘤相关细胞角蛋白 p39 (Mr 39,000) 与细胞 DNA 之间的关系已在完整细胞中进行了研究。使用 DNA-蛋白质交联技术，与顺式二氯二氨铂一起孵育，我们提供了 p39 与完整 Novikoff 腹水肝癌细胞中 DNA 关联的证据。将细胞用顺式二氯二氨铂处理并溶解在含有NaDodSO4的缓冲液中，并通过高速离心沉淀DNA。通过免疫转移分析，在交联样品的 DNA 沉淀中发现了细胞角蛋白，而对照样品中则没有细胞角蛋白。 CsCl 密度梯度离心进一步证实了这一结果。总的来说，这些结果表明细胞角蛋白-DNA 在核纤层上或核纤附近的丝结合位点处存在关联。
The relationship between the rat tumor-associated cytokeratin p39 (Mr 39,000) and cellular DNA has been studied in intact cells. Using a DNA-protein crosslinking technique, incubation with cis-dichlorodiammineplatinum, we present evidence for the association of p39 with DNA in intact Novikoff ascites hepatoma cells. The cells were treated with cis-dichlorodiammineplatinum and solubilized in NaDodSO4-containing buffer, and the DNA was pelleted by high-speed centrifugation. By immunotransfer analysis, the cytokeratin was found in the DNA pellet of the crosslinked samples while absent from the controls. This result was further substantiated by CsCl density-gradient centrifugation. Collectively, these results suggest a cytokeratin-DNA association at the filament binding sites on or near the nuclear lamina.