Human papillomavirus found in sperm head of young adult males affects the progressive motility
Human papillomavirus found in sperm head of young adult males affects the progressive motility
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DOI：
10.1016/j.fertnstert.2008.10.050
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发表时间：
2010-02-01
影响因子：
6.7
通讯作者：
Palu, Giorgio
中科院分区：
文献类型：
--
作者：
Foresta, Carlo;Garolla, Andrea;Palu, Giorgio
目的：评估年轻成年男性中人乳头瘤病毒（HPV）精子感染的患病率及其与精子参数的相关性。设计：横断面临床研究。设置：大学医院的男科和微生物科。患者：一组200名年轻男性志愿者（18岁），100例有过性行为，100例无过性行为。主要结果测量：精液参数，精子HPV培养和荧光原位杂交（FISH）分析，用于检测精子头部的HPV。结果：HPV检测结果与精子参数和FISH分析结果进行比较。在100名已经进行无保护性交的年轻人中，10名受试者的精子细胞中存在HPV感染，其存在与精子活力降低有关。此外，感染的样本显示，约25%的精子有HPV DNA阳性的头部网站，但目前还不清楚它是否是整合在核或noth.Conclusion（S）：这是第一次报告估计感染的受试者的HPV阳性精子的百分比和HPV感染和精子活力之间的关联。（Fertil Steril（R）2010;93：802-6.（C）2010年由美国生殖医学学会。
Objective: To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in a cohort of young adult males.Design: Cross-sectional clinical study.Setting: Andrology and Microbiology sections at a university hospital.Patient(s): A cohort of 200 young adult male volunteers (18 years old), 100 with previous sexual intercourse and 100 without previous sexual intercourse.Main Outcome Measure(s): Seminal parameters, sperm Culture for HPV and fluorescence in situ hybridization (FISH) analysis for HPV detection in the sperm head. Statistical analysis was performed with a two-tailed Student's t-test.Result(s): Results of HPV investigation were compared to sperm parameters and results of FISH analysis. HPV infection was present in sperm cells of 10 subjects among those 100 young adults who already had unprotected intercourse and its presence was associated with reduced sperm motility. Furthermore, infected samples showed that about 25% of sperm had an HPV DNA positivity at the head site, but it is unclear whether it was integrated in the nucleus or not.Conclusion(S): This is the first report estimating the percentage of HPV-positive sperm in infected subjects and the association between HPV infection and sperm motility. (Fertil Steril (R) 2010;93:802-6. (C)2010 by American Society for Reproductive Medicine.)