The transcriptome of Toxoplasma gondii.
The transcriptome of Toxoplasma gondii.
复制标题
弓形虫的转录组。
DOI：
10.1186/1741-7007-3-26
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发表时间：
2005-12-02
期刊：
影响因子：
5.4
通讯作者：
White MW
中科院分区：
文献类型：
--
作者：
Radke JR;Behnke MS;Mackey AJ;Radke JB;Roos DS;White MW
弓形虫病是人类和动物最常见的寄生虫病之一。弓形虫病是一种慢性病，由速殖子阶段转变为潜伏的缓殖子-包囊形式，并导致免疫系统受损的个体终生有复发的风险。鉴于组织囊肿形成的重要性，研究缓殖子分化的方法的发展一直是人们关注的焦点，尽管发育开关的分子基础在很大程度上仍然是未知的。我们已经使用了基因表达的系列分析（SAGE），以确定弓形虫的中间宿主的生活周期，导致形成的缓殖子/组织囊肿的转录组。通过来自9个不同文库（约300，000个SAGE标签）的>4倍覆盖率提供了基因表达的广泛观点，这些文库代表了主要寄生虫种群和代表三种典型基因型的实验室菌株中的关键发育转变。SAGE标签，和它们相应的mRNA，进行了分析的丰度，独特性，和反义/正义极性和染色体分布和发育特异性。这项研究表明，寄生虫发育过程中的表型转变是由独特的阶段特异性mRNA标记的，占总SAGE标签的18%，在每个发育阶段的标签从1-5%不等。我们还发现弓形虫mRNA库具有独特的寄生虫特异性组成，其中1/5的转录本编码在寄生虫入侵和传播中起作用的顶复门特异性基因。发育共调节基因分散在所有弓形虫染色体，是标签代表每个丰度类，和各种生化途径表明，反式作用机制可能控制基因表达在这种寄生虫。我们观察到特异性和表达水平的mRNA的主要人群（第6天后孢子感染），发生在缓殖子的发展，这是唯一共享的毒性I-RH型实验室菌株，表明RH的发展可能会被逮捕。相比之下，来自II-Me 49 B7型和III-VEGmsj型的菌株含有对应于缓殖子基因的SAGE标签，这表明发育表达的引发可能在这些菌株完成缓殖子发育的更大能力中起作用。
Toxoplasma gondii gives rise to toxoplasmosis, among the most prevalent parasitic diseases of animals and man. Transformation of the tachzyoite stage into the latent bradyzoite-cyst form underlies chronic disease and leads to a lifetime risk of recrudescence in individuals whose immune system becomes compromised. Given the importance of tissue cyst formation, there has been intensive focus on the development of methods to study bradyzoite differentiation, although the molecular basis for the developmental switch is still largely unknown. We have used serial analysis of gene expression (SAGE) to define the Toxoplasma gondii transcriptome of the intermediate-host life cycle that leads to the formation of the bradyzoite/tissue cyst. A broad view of gene expression is provided by >4-fold coverage from nine distinct libraries (~300,000 SAGE tags) representing key developmental transitions in primary parasite populations and in laboratory strains representing the three canonical genotypes. SAGE tags, and their corresponding mRNAs, were analyzed with respect to abundance, uniqueness, and antisense/sense polarity and chromosome distribution and developmental specificity. This study demonstrates that phenotypic transitions during parasite development were marked by unique stage-specific mRNAs that accounted for 18% of the total SAGE tags and varied from 1–5% of the tags in each developmental stage. We have also found that Toxoplasma mRNA pools have a unique parasite-specific composition with 1 in 5 transcripts encoding Apicomplexa-specific genes functioning in parasite invasion and transmission. Developmentally co-regulated genes were dispersed across all Toxoplasma chromosomes, as were tags representing each abundance class, and a variety of biochemical pathways indicating that trans-acting mechanisms likely control gene expression in this parasite. We observed distinct similarities in the specificity and expression levels of mRNAs in primary populations (Day-6 post-sporozoite infection) that occur prior to the onset of bradyzoite development that were uniquely shared with the virulent Type I-RH laboratory strain suggesting that development of RH may be arrested. By contrast, strains from Type II-Me49B7 and Type III-VEGmsj contain SAGE tags corresponding to bradyzoite genes, which suggests that priming of developmental expression likely plays a role in the greater capacity of these strains to complete bradyzoite development.
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影响因子：
4.4
作者：
Callebaut, I;Prat, K;Meurice, E;Mornon, JP;Tomavo, S
通讯作者：
Tomavo, S
影响因子：
1.5
作者：
Bohne, W;Wirsing, A;Gross, U
通讯作者：
Gross, U
影响因子：
3.2
作者：
BOEHLKE, KW;FRIESEN, JD
通讯作者：
FRIESEN, JD
影响因子：
3.1
作者：
BOHNE, W;HEESEMANN, J;GROSS, U
通讯作者：
GROSS, U
影响因子：
7
作者：
Coulson, RMR;Hall, N;Ouzounis, CA
通讯作者：
Ouzounis, CA