Insulin receptors regulate the fecundity of Nilaparvata lugens (Stal) (Hemiptera: Delphacidae)
Insulin receptors regulate the fecundity of Nilaparvata lugens (Stal) (Hemiptera: Delphacidae)
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胰岛素受体调节褐飞虱 (Stàl) 的繁殖力（半翅目：飞虱科）
DOI：
10.1016/j.aspen.2020.09.011
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发表时间：
2020-12-01
影响因子：
1.5
通讯作者：
Tang, Bin
中科院分区：
文献类型：
--
作者：
Liu, Yong-Kang;Luo, Yu-Jia;Tang, Bin
已鉴定出两个胰岛素受体（MR）基因。在这项研究中，我们采用RNA干扰（RNAi）技术来研究MR基因在N. lugens的繁殖力中的作用。用dsInR1和dsInR2 （dsInRs）混合注射同时沉默NLMR1和NLMR2基因的表达。结果表明，对NLMR1和NLMR2的RNAi均可使褐家蚕产卵量减少，部分卵和卵巢出现异常。此外，在幼虫RNAi处理后的第4天和第6天，卵黄蛋白原素（Vg）和卵黄蛋白原受体（VgR）的相对表达量显著降低。我们还检测了胰岛素/胰岛素样信号（IIS）相关基因在rnai处理后的幼虫中的相对表达水平，发现Chico（同源受体底物）、Akt（蛋白激酶B）、PI3K（磷酸肌肽3激酶）和PTEN（磷酸酶和紧张素同源基因）的表达水平在成虫期第4天和第6天下降，而FOXO （forkhead box 0）和GSK3（糖原合成酶激酶3）的表达水平上升。这些结果表明NLMR1和NLMR2的沉默通过IIS途径降低了N. lugens的繁殖力。
Two MR (insulin receptor) genes have been identified in the Nilaparvata lugens. In this study, we used RNA interference (RNAi) to investigate the role of MR genes in the fecundity of N. lugens. The expression of NLMR1 and NLMR2 genes was simultaneously silenced with mixture of dsInR1 and dsInR2 (dsInRs) injection. Our results showed that larvae RNAi against both NLMR1 and NLMR2 reduced the number of eggs laid by N. lugens and some eggs as well as ovaries were abnormal. In addition, the relative expression of Vg (vitellogenin) and VgR (vitellogenin receptor) was significantly reduced on the 4th and 6th days after insects treated with larvae RNAi reached the adult stage. We also determined the relative expression levels of insulin/insulin-like signaling (IIS) related genes in RNAi-treated larvae and found that the expression levels of Chico (homologous receptor substrate), Akt (protein kinase B), PI3K (phosphoinositide 3-kinase), and PTEN (phosphatase and tensin homolog) genes decreased whereas FOXO (forkhead box 0) and GSK3 (glycogen synthase kinase-3) levels increased on the 4th and 6th days after insects reached the adult stage. These results indicate that silencing of NLMR1 and NLMR2 reduces the fecundity of N. lugens through the IIS pathway.