Ezrin and EBP50 redistribute apically in rat uterine epithelial cells at the time of implantation and in response to cell contact
Ezrin and EBP50 redistribute apically in rat uterine epithelial cells at the time of implantation and in response to cell contact
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DOI：
10.1007/s00441-010-1088-z
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发表时间：
2011-02-01
影响因子：
3.6
通讯作者：
Murphy, Christopher R.
中科院分区：
文献类型：
--
作者：
Lecce, Laura;Lindsay, Laura A.;Murphy, Christopher R.
子宫上皮细胞（UECs）在准备植入囊胚时经历了广泛的形态重塑。这种重塑涉及肌动蛋白细胞骨架和包括微绒毛在内的表面结构的变化。Ezrin和Ezrin -radixin-moesin-binding protein-50-kDa （EBP50）将肌动蛋白丝连接到膜内粘附分子，是极化上皮中的重要分子。本研究首次利用免疫荧光显微镜和免疫沉淀技术描述了ezrin和EBP50在大鼠uec中的共定位和分子关联。这些蛋白也被定位于大鼠妊娠早期子宫上皮细胞骨架重排以及对立上皮细胞之间的顶端表面接触、囊胚接触和与硅丝接触的影响。免疫荧光显微镜显示，ezrin和EBP50对对立上皮细胞之间的接触有反应，并在妊娠第6天顶端增加。在与硅丝接触的uec中也观察到这种顶端分布。然而，在植入腔内不存在Ezrin和EBP50，似乎是模仿白细胞-内皮细胞结合发生的事件。因此，ezrin和EBP50在uec植入大鼠时发生在顶端，并对替代囊胚（丝）做出反应，这表明这些蛋白在促进子宫接受性和囊胚-上皮粘附的细胞骨架重排中起作用。它们在植入腔内的丢失可能会导致随后的胚胎侵入。
Uterine epithelial cells (UECs) undergo extensive morphological remodelling in preparation for an implanting blastocyst. This remodelling involves changes in the actin cytoskeleton and surface structures including microvilli. Ezrin and ezrin-radixin-moesin-binding protein-50-kDa (EBP50) link actin filaments to intra-membranous adhesion molecules and are important molecules in polarised epithelia. The current study is the first to describe the colocalisation and molecular association of ezrin and EBP50 in rat UECs by using immunofluorescence microscopy and immunoprecipitation techniques. These proteins have also been localised in relation to uterine epithelial cytoskeletal rearrangement during early pregnancy in the rat and to the effect of apical surface contact between opposing epithelial cells, blastocyst contact and contact with a silicon filament. Immunofluorescence microscopy has revealed that ezrin and EBP50 respond to contact between opposing epithelial cells and increase apically on day 6 of pregnancy. This apical distribution is also observed in UECs in contact with a silicon filament. Ezrin and EBP50 are however absent within the implantation chamber itself, seemingly mimicking the events that take place in leucocyte-endothelium binding. Thus, ezrin and EBP50 occur apically in UECs at the time of implantation in the rat and in response to a substitute blastocyst (filament) suggesting a role for these proteins in the cytoskeletal rearrangements that facilitate uterine receptivity and blastocyst-epithelial adhesion. Their loss within the implantation chamber possibly allows the subsequent invasion of the embryo.