Calbindin-D28k (CaBP28k) identification and regulation by 1,25-dihydroxyvitamin D3 in human choriocarcinorna cell line JEG-3
Calbindin-D28k (CaBP28k) identification and regulation by 1,25-dihydroxyvitamin D3 in human choriocarcinorna cell line JEG-3
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DOI：
10.1016/j.mce.2005.03.002
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发表时间：
2005-05-31
影响因子：
4.1
通讯作者：
Lafond, J
中科院分区：
文献类型：
--
作者：
Belkacemi, L;Züegel, U;Lafond, J
钙结合蛋白D28 k（Calbindin-D28 k，CaBP 28 k）是一种细胞内钙离子结合蛋白，在肠、肾、胎盘等组织中表达。这种蛋白质被认为与Ca 2（+）稳态有关。虽然众所周知，CaBP 28 k在肠和肾中受到1，25-二羟基维生素D-3[1，25（OH）（2）D-3]的影响，但关于该蛋白在人胎盘滋养层中的调节还一无所知。本研究采用JEG-3细胞系研究了1，25（OH）（2）D-3对CaBP 28 k与1，25（OH）（2）D-3受体（VDR）的调节作用。我们的数据首次证明了CaBP 28 k mRNA和蛋白质都被1，25（OH）（2）D-3以剂量依赖的方式高度诱导。此外，CaBP 28 k和VDR mRNA表达的增加和随后的减少表明了响应于1，25（OH）（2）D-3的基因表达变化的瞬时性质。这与CaBP 28 k和VDR蛋白质增加的时间模式形成对比。我们还表明，新的RNA和蛋白质的合成需要1，25（OH）（2）D-3诱导的CaBP 28 k上调。此外，1，25（OH）（2）D-3的25-羧酸酯类似物ZK 159222用作1，25（OH）（2）D-3信号传导的拮抗剂，证实1，25（OH）（2）D-3确实涉及CaBP 28 k的诱导。这些新的发现是对驱动人胎盘中CaBP 28 k表达调控的过程的贡献。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Calbindin-D28k (CaBP28k) is a cytosolic calcium (Ca2(+))-binding protein expressed ill tissues such as intestine, kidneys and placenta. This protein is thought to be involved in Ca2(+) homeostasis. While it is well known that CaBP28k is influenced by 1,25-dihydroxyvitamin D-3[1,25(OH)(2)D-3] in the intestine and kidneys, nothing is known regarding the regulation of this protein in trophoblasts of human placenta. We used JEG-3 syncytiotrophoblast-like carcinoma cell line to study the regulation of CaBP28k in correlation with 1,25(OH)(2)D-3 receptor (VDR) following 1,25(OH)(2)D-3 treatments. Our data demonstrated for the first time that both CaBP28k mRNA and protein were highly induced by the addition of 1,25(OH)(2)D-3 in dose-dependent manner. Moreover, the increase and subsequent decrease in the expression of CaBP28k and VDR mRNAs indicates the transient nature of the changes in gene expression in response to 1,25(OH)(2)D-3. This is in contrast with the temporal pattern of increasing protein for CaBP28k and VDR. We also showed that new RNA and protein syntheses are required for 1,25(OH)(2)D-3-induced upregulation of CaBP28k. Furthermore, a 25-carboxylic ester analogue of 1,25(OH)(2)D-3, ZK159222, used as an antagonist of 1,25(OH)(2)D-3 signaling confirmed that indeed 1,25(OH)(2)D-3 was implicated in the induction of CaBP28k. These novel findings are a contribution to the processes that drive CaBP28k expression regulation in human placenta. (c) 2005 Elsevier Ireland Ltd. All rights reserved.