CAMP-INDUCED CHANGES OF INTRACELLULAR FREE MG2+ LEVELS IN HUMAN ERYTHROCYTES
CAMP-INDUCED CHANGES OF INTRACELLULAR FREE MG2+ LEVELS IN HUMAN ERYTHROCYTES
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DOI：
10.1016/0167-4889(93)90093-5
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发表时间：
1993-12-16
期刊：
影响因子：
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通讯作者：
MORISHIMA, I
中科院分区：
文献类型：
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作者：
MATSUURA, T;KANAYAMA, Y;MORISHIMA, I
为了研究cAMP在调节细胞内游离镁浓度（[Mg~（2+）] i）中的作用，我们用P-31-NMR光谱测量了人红细胞中的[Mg~（2+）] i。（-）-异丙肾上腺素、毛喉素、Bt2cAMP和8-溴-cAMP降低人红细胞[Mg~（2+）] i。Bt2cAMP不增加红细胞Mg~（2+）流出率。HA1004是cAMP依赖性激酶的有效抑制剂，在无Mg~（2+）缓冲溶液中显著增加[Mg~（2+）] i。加入8-溴-cGMP或12-O-十四酰佛波醇13-乙酸酯（TPA）对[Mg~（2+）] i无影响。这些结果表明β-肾上腺素能刺激和cAMP在调节人红细胞[Mg~（2+）] i中起重要作用。
To examine the role of cAMP in the regulation of intracellular free magnesium concentration ([Mg2+]i), we measured [Mg2+]i in human erythrocytes by P-31-NMR spectroscopy. (-)-Isoproterenol, forskolin, Bt2cAMP and 8-bromo-cAMP decreased [Mg2+]i in human erythrocytes. Bt2cAMP did not increase the effux rate of Mg2+ from erythrocytes. HA1004, a potent inhibitor of cAMP-dependent kinases, markedly increased the [Mg2+]i in a Mg2+-free buffer solution. Addition of 8-bromo-cGMP or 12-O-tetradecanoylphorbol 13-acetate (TPA) did not affect the [Mg2+]i. These results suggest that beta-adrenergic stimulation and cAMP play an important role in the regulation of [Mg2+]i in human erythrocytes.