HIGH-LEVEL EXPRESSION OF INTRODUCED CHIMAERIC GENES IN REGENERATED TRANSFORMED PLANTS
HIGH-LEVEL EXPRESSION OF INTRODUCED CHIMAERIC GENES IN REGENERATED TRANSFORMED PLANTS
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DOI：
10.1002/j.1460-2075.1985.tb03949.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
BEDBROOK, J
中科院分区：
文献类型：
--
作者：
JONES, JDG;DUNSMUIR, P;BEDBROOK, J
将来自矮牵牛叶绿素a/B结合蛋白基因的启动子DNA序列与章鱼碱合酶DNA序列融合，并将所得嵌合基因引入矮牵牛和烟草细胞中。检测含有嵌合基因的转化的再生矮牵牛植物群体，以便可以在独立的转化体之间比较任何特定构建体的表达。在嵌合基因表达水平的转化体之间观察到实质性变化。一般而言，其中接头序列中断5“-非翻译区的转录融合物比翻译融合物产生更少的嵌合mRNA积累。在表达最活跃的转化体中，来自引入的嵌合基因的mRNA的量是内源性野生型基因的一半。在嵌合基因和内源基因的同一位置开始转录。翻译的cab/ocs融合体的构建引起章鱼碱合酶蛋白中的三个氨基酸的变化，并且在嵌合基因的mRNA大量表达的植物中不存在功能性章鱼碱合酶。
Promoter DNA sequences from a petunia chlorophyll a/b binding protein gene were fused to octopine synthase DNA sequences and the resulting chimaeric genes were introduced into petunia and tobacco cells. Populations of transformed regenerated petunia plants containing the chimaeric genes were examined so that the expression of any particular construction could be compared between independent transformants. Substantial variation was observed between transformants in the level of chimaeric gene expression. In general, transcriptional fusions in which a linker sequence interrupted the 5''-untranslated region gave rise to less chimaeric mRNA accumulation than a translational fusion. In the most actively expressing transformants the amount of mRNA from the introduced chimaeric genes was half that of the endogenous wild-type gene. Transcription initiated at the same place in the chimaeric and endogenous genes. Construction of the translational cab/ocs fusion caused three amino acid changes in the octopine synthase protein and functional octopine synthase enzyme was absent from plants in which mRNA for the chimaeric gene was abundantly expressed.