Proteomic profiling of eccrine sweat reveals its potential as a diagnostic biofluid for active tuberculosis
Proteomic profiling of eccrine sweat reveals its potential as a diagnostic biofluid for active tuberculosis
复制标题
DOI：
10.1002/prca.201500071
复制
发表时间：
2016-05-01
影响因子：
2
通讯作者：
Prenni, Jessica E.
中科院分区：
文献类型：
--
作者：
Adewole, Olanisun Olufemi;Erhabor, Greg Efosa;Prenni, Jessica E.
目的：出汗过多是该疾病的常见症状，也是一种尚未开发的用于结核病诊断的生物液；我们进行了一项概念验证研究，以确定小汗腺汗液中活动性结核病的潜在诊断生物标志物。实验设计：我们对从活动性肺结核、其他肺部疾病（非结核病）和健康对照患者中采集的小汗腺汗液样本进行了整体蛋白质组学分析。对活动性结核病、非结核病和健康对照者之间的蛋白质组学进行比较，以寻找活动性结核病的潜在生物标志物。结果：汇集了 32 名活动性结核病患者、27 名非结核病患者和 24 名表面健康对照者的汗液样本，全部均为 HIV 阴性。在所有三组的小汗腺汗液中都鉴定出了 100 多种独特的蛋白质。仅在活动性结核病患者的汗液中检测到了 26 种蛋白质，而其余检测到的蛋白质在三组之间重叠。基因本体论评估表明，在活动性结核病患者的汗液中独特检测到的蛋白质参与免疫反应和辅助蛋白质转运。仅在活动性结核病患者中检测到细胞成分（例如核糖体）的基因产物。数据可通过 ProteomeXchange 获得，标识符为 PXD003224。 结论和临床相关性：活动性结核病患者汗液的蛋白质组学是生物标志物鉴定的可行方法，可用于开发基于非痰液的检测活动性结核病的测试。
Purpose: Excessive sweating is a common symptom of the disease and an unexplored biofluid for TB diagnosis; we conducted a proof-of-concept study to identify potential diagnostic biomarkers of active TB in eccrine sweat.Experimental design: We performed a global proteomic profile of eccrine sweat sampled from patients with active pulmonary TB, other lung diseases (non-TB disease), and healthy controls. A comparison of proteomics between Active-TB, Non-TB, and Healthy Controls was done in search for potential biomarkers of active TB.Results: Sweat specimens were pooled from 32 active TB patients, 27 patients with non-TB diseases, and 24 apparently healthy controls, all were negative for HIV. Over 100 unique proteins were identified in the eccrine sweat of all three groups. Twenty-six proteins were exclusively detected in the sweat of patients with active TB while the remaining detected proteins overlapped between three groups. Gene ontology evaluation indicated that the proteins detected uniquely in sweat of active TB patients were involved in immune response and auxiliary protein transport. Gene products for cellular components (e.g. ribosomes) were detected only in active TB patients. Data are available via ProteomeXchange with identifier PXD003224.Conclusions and clinical relevance: Proteomics of sweat from active TB patients is a viable approach for biomarker identification, which could be used to develop a nonsputum-based test for detection of active TB.