Molecular features distinguish ten neuronal types in the mouse superficial superior colliculus.
Molecular features distinguish ten neuronal types in the mouse superficial superior colliculus.
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DOI：
10.1002/cne.23952
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发表时间：
2016-08-01
期刊：
影响因子：
--
通讯作者：
Kim IJ
中科院分区：
文献类型：
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作者：
Byun H;Kwon S;Ahn HJ;Liu H;Forrest D;Demb JB;Kim IJ
上丘（SC）是一个中脑中枢，参与控制头部和眼睛的运动，以响应来自多种感觉模式的输入。视觉输入来自视网膜和视觉皮层，并汇聚到SC （sSC）的浅层。sSC中的神经元将信息发送到更深层的SC和调节视觉引导行为的丘脑核。目前，由于缺乏定义特定细胞类型的分子标记，我们对sSC神经元的理解受到阻碍。为了更好地了解sSC神经元的身份和组织，我们采用系统的方法研究了转录因子、细胞粘附分子、神经肽和钙结合蛋白四个分子家族的基因表达。在小鼠sSC中，我们发现了12个具有不同表达模式的分子：钙粘蛋白7、接触蛋白3、netrin G2、钙粘蛋白6、原钙粘蛋白20、类维甲酸相关孤儿受体β、脑特异性同源盒/POU结构域蛋白3b、Ets变异基因1、P物质、生长抑素、血管活性肠多肽和小蛋白。双标记实验，通过原位杂交或免疫染色，表明12个分子标记共同定义了10种不同的sSC神经元类型。这些细胞类型的特征位置将sSC分为4个不同的层。这里确定的12个标记将作为有价值的工具来研究调节sSC神经元类型发育的分子机制。这些标记也可以用来检查形成视网膜丘、皮质丘或丘丘脑通路的特定细胞类型之间的联系。
The superior colliculus (SC) is a midbrain center involved in controlling head and eye movements in response to inputs from multiple sensory modalities. Visual inputs arise from both the retina and visual cortex and converge onto the superficial layer of the SC (sSC). Neurons in sSC send information to deeper layers of SC and to thalamic nuclei that modulate visually guided behaviors. Presently, our understanding of sSC neurons is impeded by a lack of molecular markers that define specific cell types. To better understand the identity and organization of sSC neurons, we took a systematic approach to investigate gene expression within four molecular families: transcription factors, cell adhesion molecules, neuropeptides and calcium binding proteins. Our analysis revealed 12 molecules with distinct expression patterns in mouse sSC: cadherin 7, contactin 3, netrin G2, cadherin 6, protocadherin 20, retinoid-related orphan receptor β, brain-specific homeobox/POU domain protein 3b, Ets variant gene 1, substance P, somatostatin, vasoactive intestinal polypeptide and parvalbumin. Double labeling experiments, by either in situ hybridization or immunostaining, demonstrated that the 12 molecular markers collectively define 10 different sSC neuronal types. The characteristic positions of these cell types divide sSC into 4 distinct layers. The 12 markers identified here will serve as valuable tools to examine molecular mechanisms that regulate development of sSC neuron types. These markers could also be used to examine the connections between specific cell types that form retinocollicular, corticocollicular or colliculothalamic pathways.