Identification of Hydroxycinnamoylquinic Acids of Arnica Flowers and Burdock Roots Using a Standardized LC-DAD-ESI/MS Profiling Method
Identification of Hydroxycinnamoylquinic Acids of Arnica Flowers and Burdock Roots Using a Standardized LC-DAD-ESI/MS Profiling Method
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DOI：
10.1021/jf802412m
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发表时间：
2008-11-12
影响因子：
6.1
通讯作者：
Harnly, James M.
中科院分区：
文献类型：
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作者：
Lin, Long-Ze;Harnly, James M.
采用LC-DAID-ESVIVIS建立了一种基于标准品直接比较的常用羟基肉桂酰奎尼酸的筛选方法。从市售标准品、在常见植物（朝鲜蓟、芦笋、咖啡豆、金银花、甘薯和斑鸠菊叶）中明确鉴定的化合物和化学修饰的标准品组装单咖啡酰奎宁异构体、二咖啡酰奎宁异构体和三咖啡酰奎宁异构体的完整标准品组。采用基于LC-DAD-ESI/MS的标准化分析方法，对4个C18反相色谱柱上的30种酚类化合物进行了分析，并对它们的洗脱顺序和保留时间进行了评价。在标准化的液相色谱条件下，仅使用两个色谱柱，利用收集的酚类化合物数据库，就可以分离山金车花中所有的羟基肉桂酰奎尼酸缀合物，并鉴定出28和18个羟基肉桂酰奎尼酸。和牛蒡根（牛蒡），分别其中22个是首次报告。
A screening method using LC-DAID-ESVIVIS was developed for the identification of common hydroxycinnamoylquinic acids based on direct comparison with standards. A complete standard set for mono-, di-, and tricaffeoylquinic isomers was assembled from commercially available standards, positively identified compounds in common plants (artichokes, asparagus, coffee bean, honeysuckle flowers, sweet potato, and Vernonia amygdalina leaves) and chemically modified standards. Four C18 reversed phase columns were tested using the standardized profiling method (based on LC-DAD-ESI/MS) for 30 phenolic compounds, and their elution order and retention times were evaluated. Using only two columns under standardized LC condition and the collected phenolic compound database, it was possible to separate all of the hydroxycinnamoylquinic acid conjugates and to identify 28 and 18 hydroxycinnamoylquinic acids in arnica flowers (Amica montana L.) and burdock roots (Arctium lappa L.), respectively. Of these, 22 are reported for the first time.