Of Cytometry, Stem Cells and Fountain of Youth
Of Cytometry, Stem Cells and Fountain of Youth
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DOI：
10.1007/s12015-017-9733-5
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发表时间：
2017-08-01
影响因子：
4.8
通讯作者：
Darzynkiewicz, Zbigniew
中科院分区：
文献类型：
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作者：
Galkowski, Dariusz;Ratajczak, Mariusz Z.;Darzynkiewicz, Zbigniew
概述了细胞计量学应用的进展，以确定和分类干细胞，激光扫描细胞计量学和ImageStream成像仪器，以进一步分析这些细胞的形态，和大量的细胞计量学分类大量的细胞标志物在大细胞群体。综述了不同类型的干细胞，包括癌症干细胞的潜在候选者，关于它们的“干性”和其他特征。评价了在鉴定和分离“非常小的胚胎样干细胞”（VSELs）及其自体移植用于组织修复和老年保护方面的进一步进展。还评估了透明质酸作为干细胞的监护人和控制者的功能，透明质酸是干细胞的主要生态位成分。简要评价了干细胞在再生医学和肿瘤学中应用的最新进展和挑战，以及它们在不同医学学科（包括老年医学）中的未来作用。
Outlined are advances of cytometry applications to identify and sort stem cells, of laser scanning cytometry and ImageStream imaging instrumentation to further analyze morphometry of these cells, and of mass cytometry to classify a multitude of cellular markers in large cell populations. Reviewed are different types of stem cells, including potential candidates for cancer stem cells, with respect to their "stemness", and other characteristics. Appraised is further progress in identification and isolation of the "very small embryonic-like stem cells" (VSELs) and their autogenous transplantation for tissue repair and geroprotection. Also assessed is a function of hyaluronic acid, the major stem cells niche component, as a guardian and controller of stem cells. Briefly appraised are recent advances and challenges in the application of stem cells in regenerative medicine and oncology and their future role in different disciplines of medicine, including geriatrics.