Seasonal variation of prolactin in plasma of male goats.
Seasonal variation of prolactin in plasma of male goats.
复制标题
雄性山羊血浆中催乳素的季节变化。
DOI：
10.1530/jrf.0.0370095
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
H. Buttle
中科院分区：
文献类型：
--
作者：
H. Buttle
在研究不同生理状态山羊泌乳素分泌的过程中，对公山羊血浆泌乳素浓度进行了全年测定。在取样期开始时，使用了3只年龄为5\m=1/2\（Miljohn）、2\m=1/2\（鲁珀特）和1\m=1/2\（Minto）岁的完整公山羊。山羊在通常的饲养条件下饲养，夏季放牧，冬季饲养在单独的公山羊舍中的单独围栏中;必要时使用红外线灯进行辅助加热。在繁殖季节，所有三只雄性都被用来与牛群中的雌性交配，所有三只雄性都能生育。每周一次从颈静脉抽取血液样本，时间为14.30至15.30小时，为期15个月。通过静脉穿刺将血液样品采集到肝素化注射器中，以1800 g在4 ℃下离心20 min，并将血浆储存在-20 ℃下直至需要用于测定。第二年（1972年），在6月22日至23日和12月19日至20日，每小时对相同的3只完整雄性和另1只1岁大的去势雄性以及4只去势雄性进行24小时采样。在开始采样前，所有动物均被限制在单独的畜栏中1周。采样前一天，将聚乙烯插管插入颈静脉。尽可能少地使用夜间取样所需的辅助照明。
In the course of studies on the secretion of prolactin by goats in different physiological states, the concentrations of prolactin in the plasma of male goats were measured throughout the year. Three intact male goats aged 5\m=1/2\ (Miljohn), 2\m=1/2\ (Rupert) and 1\m=1/2\ (Minto) years at the beginning of the sampling period were used. The goats were main-tained under the usual husbandry conditions, being turned out to pasture in the summer and kept in individual pens in a separate male goat house in winter; supplementary heating from infra-red lamps was used when necessary. During the breeding season, all three males were used to mate with females in the herd and all three were fertile. Samples of blood were withdrawn from a jugular vein once a week, between 14.30 and 15.30 hours for a period of 15 months. The blood samples were taken by venepuncture into heparinized syringes, centri-fuged at 4\s=deg\Cfor 20 min at 1800 g and the plasma was stored at \m=-\20\s=deg\C until required for assay. The following year (1972), the same three intact males and another, Ulysses, aged 1 year, and also four castrated males were sampled hourly for 24 hr on 22nd to 23rd June and again on 19th to 20th December. All animals were confined to individual pens for 1 week before sampling started. The day before sampling, polyethylene cannulae were inserted into a jugular vein. Supplemen¬ tary lighting needed to take samples at night was used as little as possible.